Antioxidant properties of blirubin in the model organism, Caenorhabditis elegans.

Danny McCaughan, Catherine Au, Alexandre Benedetto, Dejan Milatovic, Judy Aschner, Michael Aschner
{"title":"Antioxidant properties of blirubin in the model organism, <i>Caenorhabditis elegans</i>.","authors":"Danny McCaughan,&nbsp;Catherine Au,&nbsp;Alexandre Benedetto,&nbsp;Dejan Milatovic,&nbsp;Judy Aschner,&nbsp;Michael Aschner","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>We evaluated the effects of bilirubin on the nematode, <i>C. elegans</i>, specifically addressing the ability of bilirubin to induce oxidative stress and alter glutathione (GSH) content as measures of injury. Bilirubin exposure caused a doubling of the spectrophotometric absorption at 440 nm in wild-type <i>C. elegans</i> irrespective of the bilirubin concentration used, suggesting that bilirubin is readily taken up by the worms. No changes were noted in growth, phenotype or reproductive cycle at any bilirubin concentration at 24, 48, and 72 hrs. The oxidative stress inducible green fluorescent protein (GFP) expression in the <i>gst-4::GFP</i> strain was decreased upon exposure to bilirubin at a concentration of 0.5 mM at 24, 48 and 72 hrs. This trend was not statistically significant at 24 or 72 hrs, but did reach statistical significance at 48 hours (p < 0.05). Glutathione (GSH) content in the <i>gst-4::GFP</i> strain showed a significant increase as early as 20 hours post treatment with 0.5 mM bilirubin (p < 0.05). Microarray analysis showed that in bilirubin-exposed worms, 27 genes were up-regulated, and 90 genes were down-regulated (by >1.3 fold vs. controls). The transcription factor <i>asc-1</i> was induced, whereas genes involved in transcription, trafficking and mitochondrial function were down-regulated. Our findings corroborate earlier findings of bilirubin's ability to act as an antioxidant, most likely by reducing the metabolic requirement in <i>C. elegans</i>.</p>","PeriodicalId":14115,"journal":{"name":"International journal of neuroprotection and neuroregeneration","volume":"4 3","pages":"252-262"},"PeriodicalIF":0.0000,"publicationDate":"2008-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6555407/pdf/nihms-1004066.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International journal of neuroprotection and neuroregeneration","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

We evaluated the effects of bilirubin on the nematode, C. elegans, specifically addressing the ability of bilirubin to induce oxidative stress and alter glutathione (GSH) content as measures of injury. Bilirubin exposure caused a doubling of the spectrophotometric absorption at 440 nm in wild-type C. elegans irrespective of the bilirubin concentration used, suggesting that bilirubin is readily taken up by the worms. No changes were noted in growth, phenotype or reproductive cycle at any bilirubin concentration at 24, 48, and 72 hrs. The oxidative stress inducible green fluorescent protein (GFP) expression in the gst-4::GFP strain was decreased upon exposure to bilirubin at a concentration of 0.5 mM at 24, 48 and 72 hrs. This trend was not statistically significant at 24 or 72 hrs, but did reach statistical significance at 48 hours (p < 0.05). Glutathione (GSH) content in the gst-4::GFP strain showed a significant increase as early as 20 hours post treatment with 0.5 mM bilirubin (p < 0.05). Microarray analysis showed that in bilirubin-exposed worms, 27 genes were up-regulated, and 90 genes were down-regulated (by >1.3 fold vs. controls). The transcription factor asc-1 was induced, whereas genes involved in transcription, trafficking and mitochondrial function were down-regulated. Our findings corroborate earlier findings of bilirubin's ability to act as an antioxidant, most likely by reducing the metabolic requirement in C. elegans.

模式生物秀丽隐杆线虫中胆红素的抗氧化特性。
我们评估了胆红素对线虫的影响,秀丽隐杆线虫,特别解决胆红素诱导氧化应激和改变谷胱甘肽(GSH)含量作为损伤措施的能力。无论胆红素浓度如何,暴露在野生型秀丽隐杆线虫中,在440 nm处的分光光度吸收增加一倍,这表明胆红素很容易被蠕虫吸收。在24、48和72小时的任何胆红素浓度下,没有发现生长、表型或生殖周期的变化。在0.5 mM浓度的胆红素作用下,gst-4::GFP菌株的氧化应激诱导型绿色荧光蛋白(GFP)在24、48和72小时的表达均有所降低。这一趋势在24、72 h时无统计学意义,但在48 h时达到统计学意义(p < 0.05)。0.5 mM胆红素处理后20 h, gst-4::GFP菌株谷胱甘肽含量显著升高(p < 0.05)。微阵列分析显示,在胆红素暴露的蠕虫中,27个基因上调,90个基因下调(与对照组相比,下调幅度>1.3倍)。转录因子asc-1被诱导,而参与转录、运输和线粒体功能的基因被下调。我们的发现证实了早期胆红素作为抗氧化剂的能力,最有可能通过减少秀丽隐杆线虫的代谢需求。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信