A Pilot Study to Investigate the Balance between Proteases and α1-Antitrypsin in Bronchoalveolar Lavage Fluid of Lung Transplant Recipients.

Q2 Biochemistry, Genetics and Molecular Biology

High-Throughput Pub Date : 2019-02-13 DOI:10.3390/ht8010005

Maddalena Cagnone, Davide Piloni, Ilaria Ferrarotti, Monica Di Venere, Simona Viglio, Sara Magni, Anna Bardoni, Roberta Salvini, Marco Fumagalli, Paolo Iadarola, Sabrina Martinello, Federica Meloni

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引用次数: 6

Abstract

: The neutrophilic component in bronchiolitis obliterans syndrome (BOS, the main form of chronic lung rejection), plays a crucial role in the pathogenesis and maintenance of the disorder. Human Neutrophil Elastase (HNE), a serine protease responsible of elastin degradation whose action is counteracted by α1-antitrypsin (AAT), a serum inhibitor specific for this protease. This work aimed to investigate the relationship between HNE and AAT in bronchoalveolar lavage fluid (BALf) from stable lung transplant recipients and BOS patients to understand whether the imbalance between proteases and inhibitors is relevant to the development of BOS. To reach this goal a multidisciplinary procedure was applied which included: (i) the use of electrophoresis/western blotting coupled with liquid chromatography-mass spectrometric analysis; (ii) the functional evaluation of the residual antiprotease activity, and (iii) a neutrophil count.

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肺移植受者支气管肺泡灌洗液中蛋白酶和α1-抗胰蛋白酶平衡的初步研究

闭塞性细支气管炎综合征(BOS)是慢性肺排斥反应的主要形式，其中性粒细胞成分在该病的发病和维持中起着至关重要的作用。人中性粒细胞弹性酶(HNE)，一种负责弹性蛋白降解的丝氨酸蛋白酶，其作用被α1-抗胰蛋白酶(AAT)抵消，α1-抗胰蛋白酶(AAT)是该蛋白酶的特异性血清抑制剂。本研究旨在探讨稳定肺移植受者和BOS患者支气管肺泡灌洗液(BALf)中HNE和AAT的关系，以了解蛋白酶和抑制剂之间的失衡是否与BOS的发生有关。为了实现这一目标，应用了多学科程序，其中包括:(i)使用电泳/western blotting结合液相色谱-质谱分析;(ii)剩余抗蛋白酶活性的功能评估，以及(iii)中性粒细胞计数。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

High-Throughput Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

3.60

自引率

0.00%

发文量

审稿时长

9 weeks

期刊介绍： High-Throughput (formerly Microarrays, ISSN 2076-3905) is a multidisciplinary peer-reviewed scientific journal that provides an advanced forum for the publication of studies reporting high-dimensional approaches and developments in Life Sciences, Chemistry and related fields. Our aim is to encourage scientists to publish their experimental and theoretical results based on high-throughput techniques as well as computational and statistical tools for data analysis and interpretation. The full experimental or methodological details must be provided so that the results can be reproduced. There is no restriction on the length of the papers. High-Throughput invites submissions covering several topics, including, but not limited to: -Microarrays -DNA Sequencing -RNA Sequencing -Protein Identification and Quantification -Cell-based Approaches -Omics Technologies -Imaging -Bioinformatics -Computational Biology/Chemistry -Statistics -Integrative Omics -Drug Discovery and Development -Microfluidics -Lab-on-a-chip -Data Mining -Databases -Multiplex Assays