Housing Gnotobiotic Mice in Conventional Animal Facilities

Q1 Agricultural and Biological Sciences

Current protocols in mouse biology Pub Date : 2019-01-15 DOI:10.1002/cpmo.59

Máximo E. Lange, Richard R. E. Uwiera, G. Douglas Inglis

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引用次数: 9

Abstract

Gnotobiotic mice are an established, robust model utilized in current research to study host-microbiota interactions. For years isolators have been used to rear germ-free and gnotobiotic mice. However, isolators can be costly and the segregation of treatments within the same isolator is problematic. Recently, methodologies for housing germ-free mice in specially designed individually ventilated cages (IVCs) operated under barrier mode have been developed; however, this equipment is costly and its operation in barrier mode for research involving germ-free mice and pathogens is not permissible under modern biosafety and biosecurity regulations. This article describes a method to house germ-free mice in a commonly available conventional IVC system operated under containment mode. This technique allows researchers to maintain the germ-free or gnotobiotic status of mice tested up to 4 weeks with weekly handling while working with pathogens using each IVC as a separate experimental unit. © 2019 Her Majesty the Queen in Right of Canada.

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在传统动物设施中饲养非生物小鼠

灵生小鼠是目前研究宿主-微生物群相互作用的一种成熟、稳健的模型。多年来，分离器一直用于培养无菌和无菌小鼠。然而，隔离器可能很昂贵，并且在同一隔离器内隔离治疗是有问题的。最近，开发了在屏障模式下运行的专门设计的单独通风笼(IVCs)中饲养无菌小鼠的方法;然而，该设备价格昂贵，并且在涉及无菌小鼠和病原体的研究中以屏障模式操作在现代生物安全和生物安全法规下是不允许的。本文描述了一种在封闭模式下运行的常规IVC系统中饲养无菌小鼠的方法。这项技术使研究人员能够在使用每个IVC作为单独的实验单元处理病原体的同时，在每周处理的情况下，将测试小鼠的无菌或非生物状态保持长达4周。©2019加拿大女王陛下。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Current protocols in mouse biology Agricultural and Biological Sciences-Animal Science and Zoology

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期刊介绍： Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet, all too often, nuances that are critical for an experiment''s success are not captured in the primary literature but exist only as part of a lab''s oral tradition. The aim of Current Protocols in Mouse Biology is to provide the clearest, most detailed and reliable step-by-step instructions for protocols involving the use of mice in biomedical research. Written by experts in the field and extensively edited to our exacting standards, the protocols include all of the information necessary to complete an experiment in the laboratory—introduction, materials lists with supplier information, detailed step-by-step procedures with helpful annotations, recipes for reagents and solutions, illustrative figures and information-packed tables. Each article also provides invaluable discussions of background information, applications of the methods, important assumptions, key parameters, time considerations, and tips to help avoid common pitfalls and troubleshoot experiments. Furthermore, Current Protocols in Mouse Biology content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly issues allow Current Protocols to constantly evolve to keep pace with the newest discoveries and developments. Current Protocols in Mouse Biology brings together resources in mouse biology and genetics and provides a mouse protocol resource that covers all aspects of mouse biology. Current Protocols in Mouse Biology also permits optimization of mouse model usage, which is significantly impacted by both cost and ethical constraints. Optimal and standardized mouse protocols ultimately reduce experimental variability and reduce the number of animals used in mouse experiments.