Protective Effect of Selenium-L-methionine on Radiation-induced Acute Pneumonitis and Lung Fibrosis in Rat.

IF 3.2 Q2 Pharmacology, Toxicology and Pharmaceutics

Current clinical pharmacology Pub Date : 2019-01-01 DOI:10.2174/1574884714666181214101917

Peyman Amini, Sedighe Kolivand, Hana Saffar, Saeed Rezapoor, Elahe Motevaseli, Masoud Najafi, Farzad Nouruzi, Dheyauldeen Shabeeb, Ahmed Eleojo Musa

{"title":"Protective Effect of Selenium-L-methionine on Radiation-induced Acute Pneumonitis and Lung Fibrosis in Rat.","authors":"Peyman Amini, Sedighe Kolivand, Hana Saffar, Saeed Rezapoor, Elahe Motevaseli, Masoud Najafi, Farzad Nouruzi, Dheyauldeen Shabeeb, Ahmed Eleojo Musa","doi":"10.2174/1574884714666181214101917","DOIUrl":null,"url":null,"abstract":"Background: In this study, we aimed to detect the changes in the level of interleukin (IL)-4 and IL-13 cytokines and their downstream genes including interleukin-13 receptor subunit alpha-2 (IL13Ra2), interleukin-4 receptor subunit alpha-1 (IL4Ra1), dual oxidase 1 (DUOX1) and dual oxidase 2 (DUOX2). The protective effects of Selenium-L-methionine on radiation-induced histopathological damages and changes in the level of these cytokines and genes were detected.Methods: Four groups of 20 rats (5 rats in each) namely, control; Selenium-L-methionine, radiation and radiation plus Selenium-L-methionine were used in this study. 4 mg/kg of Selenium-Lmethionine was administered 1 day before irradiation and five consecutive days after irradiation. Irradiation was done using a dose of 15 Gy 60Co gamma rays at 109 cGy/min. All rats were sacrificed 10 weeks after irradiation for detecting changes in IL-4 and IL-13 cytokines, the expressions of IL13Ra2, IL4Ra1, Duox1 and Duox2 and histopathological changes.Results: The level of IL-4 but not IL-13 increased after irradiation. This was associated with increased expression of IL4Ra1, Duox1 and Duox2, in addition to changes in morphological properties. Selenium-L-methionine could attenuate all injury markers following lung irradiation.Conclusion: Selenium-L-methionine can protect lung tissues against toxic effects of ionizing radiation. It is possible that the modulation of immune responses and redox interactions are involved in the radioprotective effect of this agent.","PeriodicalId":10746,"journal":{"name":"Current clinical pharmacology","volume":null,"pages":null},"PeriodicalIF":3.2000,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2174/1574884714666181214101917","citationCount":"14","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current clinical pharmacology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2174/1574884714666181214101917","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Pharmacology, Toxicology and Pharmaceutics","Score":null,"Total":0}

引用次数: 14

Abstract

Background: In this study, we aimed to detect the changes in the level of interleukin (IL)-4 and IL-13 cytokines and their downstream genes including interleukin-13 receptor subunit alpha-2 (IL13Ra2), interleukin-4 receptor subunit alpha-1 (IL4Ra1), dual oxidase 1 (DUOX1) and dual oxidase 2 (DUOX2). The protective effects of Selenium-L-methionine on radiation-induced histopathological damages and changes in the level of these cytokines and genes were detected.

Methods: Four groups of 20 rats (5 rats in each) namely, control; Selenium-L-methionine, radiation and radiation plus Selenium-L-methionine were used in this study. 4 mg/kg of Selenium-Lmethionine was administered 1 day before irradiation and five consecutive days after irradiation. Irradiation was done using a dose of 15 Gy 60Co gamma rays at 109 cGy/min. All rats were sacrificed 10 weeks after irradiation for detecting changes in IL-4 and IL-13 cytokines, the expressions of IL13Ra2, IL4Ra1, Duox1 and Duox2 and histopathological changes.

Results: The level of IL-4 but not IL-13 increased after irradiation. This was associated with increased expression of IL4Ra1, Duox1 and Duox2, in addition to changes in morphological properties. Selenium-L-methionine could attenuate all injury markers following lung irradiation.

Conclusion: Selenium-L-methionine can protect lung tissues against toxic effects of ionizing radiation. It is possible that the modulation of immune responses and redox interactions are involved in the radioprotective effect of this agent.

Abstract Image

查看原文本刊更多论文

硒- l -蛋氨酸对大鼠放射性急性肺炎及肺纤维化的保护作用。

背景:本研究旨在检测白细胞介素(IL)-4和IL-13细胞因子及其下游基因IL-13受体亚基α -2 (IL13Ra2)、IL -4受体亚基α -1 (IL4Ra1)、双氧化酶1 (DUOX1)和双氧化酶2 (DUOX2)水平的变化。研究了硒- l -蛋氨酸对辐射诱导的组织病理损伤的保护作用以及这些细胞因子和基因水平的变化。方法:4组大鼠20只，每组5只，分别为对照组;采用硒- l -蛋氨酸、辐射法和辐射加硒- l -蛋氨酸法进行研究。照射前1天、照射后连续5天给予硒-蛋氨酸4 mg/kg。辐照剂量为15gy 60Co伽马射线，速度为109 cGy/min。照射后10周处死大鼠，检测IL-4、IL-13细胞因子的变化，IL13Ra2、IL4Ra1、Duox1、Duox2的表达及组织病理变化。结果:辐照后血清IL-4水平升高，IL-13水平未见升高。这与IL4Ra1, Duox1和Duox2的表达增加以及形态学特性的变化有关。硒- l -蛋氨酸能减弱肺辐照后的所有损伤标志物。结论:硒- l -蛋氨酸可保护肺组织免受电离辐射的毒性作用。这可能是免疫反应的调节和氧化还原相互作用参与该剂的辐射防护作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Current clinical pharmacology PHARMACOLOGY & PHARMACY-

CiteScore

3.60

自引率

0.00%

发文量

期刊介绍： Current Clinical Pharmacology publishes frontier reviews on all the latest advances in clinical pharmacology. The journal"s aim is to publish the highest quality review articles in the field. Topics covered include: pharmacokinetics; therapeutic trials; adverse drug reactions; drug interactions; drug metabolism; pharmacoepidemiology; and drug development. The journal is essential reading for all researchers in clinical pharmacology.