Authentication of Frozen Chilean Blue Mussel (Mytilus chilensis) Commercialized in the Town of Osorno, Southern Chile, Using PCR-RFLP Analysis.

Q1 Agricultural and Biological Sciences
Nelson Colihueque, René Espinoza, Margarita Parraguez
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引用次数: 4

Abstract

Background: DNA-based technologies are reliable authentication methods for food products, enabling the detection of fraud, non-intentional substitution and control of mislabeling. The Chilean blue mussel (Mytilus chilensis) is a seafood commercialized in Chile under different formats, including packages of frozen specimens. In this format, the valves of mussels are removed during processing, thus impeding identification of the product by the consumer due to the lack of external characters.

Objective: To assess the authenticity of frozen Chilean blue mussels commercialized in southern Chile, particularly in the town of Osorno.

Methods: Six commercial brands of frozen Chilean blue mussel were authenticated by the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method, based on the analysis of an 18S rDNA fragment.

Results: Restriction patterns obtained indicate that three brands (50%) proved to be 100% authentic, given that all specimens contained in the package were Chilean blue mussels. The other three brands (50%) contained specimens of other commercial mytilids, particularly the cholga mussel (Aulacomya ater), in a variable percentage (12.5-50%).

Conclusion: This study based on the PCR-RFLP method provides evidence that Chilean blue mussels commercialized in a town located in southern Chile lack authenticity. This finding highlights the necessity for national producers to improve the production and/or packaging processes of this seafood. The authentication of commercial mussels is a matter of consumer interest and has been described in a recent patent on this issue that proposes an alternative methodology.

在智利南部Osorno镇商业化的冷冻智利蓝贻贝(Mytilus chilensis)的PCR-RFLP鉴定
背景:基于dna的技术是可靠的食品认证方法,可以检测欺诈,非故意替代和控制错标。智利蓝贻贝(Mytilus chilensis)是一种在智利以不同形式商业化的海鲜,包括冷冻标本的包装。在这种格式中,贻贝的阀门在加工过程中被移除,因此由于缺乏外部特征,阻碍了消费者对产品的识别。目的:评估在智利南部,特别是在Osorno镇商业化的冷冻智利蓝贻贝的真实性。方法:采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法对6个商业品牌的冷冻智利蓝贻贝进行鉴定,并对其中的18S rDNA片段进行分析。结果:获得的限制模式表明三个品牌(50%)被证明是100%真实的,因为包装中包含的所有标本都是智利蓝贻贝。其他三个品牌(50%)含有其他商业mytilites标本,特别是cholga贻贝(Aulacomya水),在不同的百分比(12.5-50%)。结论:本研究基于PCR-RFLP方法,提供了智利南部城镇商业化的智利蓝贻贝缺乏真实性的证据。这一发现强调了国家生产者改进这种海产品的生产和/或包装过程的必要性。商业贻贝的认证是消费者感兴趣的问题,在最近的一项专利中对此问题进行了描述,该专利提出了一种替代方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Recent patents on food, nutrition & agriculture
Recent patents on food, nutrition & agriculture Agricultural and Biological Sciences-Agronomy and Crop Science
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