Alteration of gene expression profile in CD3⁺ T-cells after downregulating MALT1.

IF 6.2 Q1 IMMUNOLOGY

ImmunoTargets and Therapy Pub Date : 2018-11-20 eCollection Date: 2018-01-01 DOI:10.2147/ITT.S179656

Xu Wang, Shuai Lu, Yankai Xiao, Ling Xu, Lingling Zhou, Junyan Hu, Bo Li, Chengwu Zeng, Yangqiu Li

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引用次数: 0

Abstract

Background: T cell immunodeficiency is a common feature in patients with different kinds of hematological disease such as T cell non-Hodgkin lymphoma (T-NHL), B cells NHL (B-NHL), NK/T cell NHL (NK/T-CL) and acute myeloid leukemia (AML). In our recent research, we found that significantly lower expression levels in MALT1 and NF-κB were related to suppression of T cell activation. Therefore, this study was conducted to further investigate the role of downregulating MALT1 in the development of immunodeficiency in T cells.

Methods: We induced activation inhibition in CD3⁺ T cells by MALT1 knockdown. Then we characterized the gene expression profile after MALT1 suppression by microarray analysis.

Result: The differentially expressed genes were ZAP-70, p65, MDM2, ATM, NFATC2 which participate in the NF-κB, p53, and NFAT pathways in CD3⁺ T cells after MALT1 downregulation.

Conclusion: MALT1 suppression may contribute to immunodeficiency in T cells via suppression of T cell activation and proliferation pathways. These data may help to explain some of the characteristics of immunodeficiency of T cells.

Abstract Image

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下调MALT1后CD3+ t细胞基因表达谱的改变。

背景:T细胞免疫缺陷是T细胞非霍奇金淋巴瘤(T-NHL)、B细胞非霍奇金淋巴瘤(B-NHL)、NK/T细胞非霍奇金淋巴瘤(NK/T- cl)和急性髓系白血病(AML)等不同血液病患者的共同特征。在我们最近的研究中，我们发现MALT1和NF-κB的表达水平显著降低与T细胞活化的抑制有关。因此，本研究旨在进一步探讨下调MALT1在T细胞免疫缺陷发展中的作用。方法:通过敲低MALT1诱导CD3+ T细胞活化抑制。然后我们通过芯片分析表征MALT1抑制后的基因表达谱。结果:MALT1下调后CD3+ T细胞中参与NF-κB、p53、NFAT通路的差异表达基因为ZAP-70、p65、MDM2、ATM、NFATC2。结论:MALT1抑制可能通过抑制T细胞活化和增殖途径导致T细胞免疫缺陷。这些数据可能有助于解释T细胞免疫缺陷的一些特征。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ImmunoTargets and Therapy IMMUNOLOGY-

CiteScore

16.50

自引率

0.00%

发文量

审稿时长

16 weeks

期刊介绍： Immuno Targets and Therapy is an international, peer-reviewed open access journal focusing on the immunological basis of diseases, potential targets for immune based therapy and treatment protocols employed to improve patient management. Basic immunology and physiology of the immune system in health, and disease will be also covered.In addition, the journal will focus on the impact of management programs and new therapeutic agents and protocols on patient perspectives such as quality of life, adherence and satisfaction.