First Detection of Chlamydia trachomatis 'Swedish' Variant (nvCT) in a Russian Couple with Infertility.

Q3 Immunology and Microbiology

Open Microbiology Journal Pub Date : 2018-10-18 eCollection Date: 2018-01-01 DOI:10.2174/1874285801812010343

Valentina Feodorova, Edgar Sultanakhmedov, Yury Saltykov, Sergey Zaitsev, Sergey Utz, Michael Corbel, Charlotte Gaydos, Thomas Quinn, Vladimir Motin

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引用次数: 0

Abstract

Background: Chronic asymptomatic chlamydial genital infection caused by the wild-type of Chlamydia trachomatis (wtCT) is the most common bacterial infection causing human infertility. The novel 'Swedish' variant of С.trachomatis (nvCT) which contains a 377 bp deletion in a region that is specifically targeted in some nucleic acid amplification tests may impede diagnosis.

Objective: The study aimed to investigate whether nvCT may be a possible cause of infertility in a couple undergoing in vitro fertilization (IVF).

Method: Clinical specimens from both genital (urethra and cervix) and extra-genital sites (pharynx, conjunctive, blood) of a couple who experienced multiple unsuccessful attempts at pregnancy by natural fertilization and IVF procedures were analyzed before and after antibiotic therapy. Both partners had neither somatic nor endocrinal abnormality nor any clinically apparent genital manifestations of Chlamydia or other STIs.

Results: Before antibiotic therapy all the samples of the Female Partner (FP) contained DNA of only the nvCT. After antibiotic therapy, additionally, DNA of wtCT of genovars E and D was detected in specimens from her conjunctiva and oropharynx. All samples of the Male Partner (MP) revealed co-infection of nvCT and wtCT. Identical SNP within the variable region 4 (VD4) of the ompA gene confirmed the identity of the wtCT strains found in both partners. The FP had a positive anti-chlamydial IgG titer. The sperm characteristics of the MP, motility (immotile spermatozoa was 51.1% versus 21.6%) and vitality (46% versus 68%) declined progressively, and the MP anti-chlamydial IgG titer was negative.

Conclusion: Infertility in this couple may have been caused by chronic asymptomatic and persistent nvCT-associated infection that was complicated by re-infection later with wtCT. This study illustrates the importance of including detection methods for nvCT strains in the investigation of infertility cases.

查看原文本刊更多论文

在一对俄罗斯不孕夫妇中首次发现沙眼衣原体 "瑞典 "变种（nvCT）。

背景：由野生型沙眼衣原体（wtCT）引起的慢性无症状生殖器衣原体感染是导致人类不孕的最常见细菌感染。沙眼衣原体的新型 "瑞典 "变异株（nvCT）在某些核酸扩增测试的特异性靶区中含有 377 bp 的缺失，可能会妨碍诊断：本研究旨在调查nvCT是否可能是一对接受体外受精（IVF）的夫妇不孕的原因：方法：对一对通过自然受精和体外受精程序多次尝试怀孕未果的夫妇在抗生素治疗前后的生殖器（尿道和宫颈）和生殖器外部位（咽部、结膜、血液）的临床标本进行分析。这对夫妇既没有躯体或内分泌异常，也没有衣原体或其他性传播感染的临床表现：结果：抗生素治疗前，女性伴侣（FP）的所有样本都只含有 nvCT 的 DNA。抗生素治疗后，在她的结膜和口咽标本中又检测到了基因 E 和 D 的 wtCT DNA。男性伴侣（MP）的所有样本均显示同时感染了 nvCT 和 wtCT。ompA基因可变区4（VD4）内相同的SNP证实了在双方中发现的wtCT菌株的身份。FP的抗衣原体IgG滴度呈阳性。MP的精子特征、运动能力（无运动精子为51.1%对21.6%）和活力（46%对68%）逐渐下降，MP的抗衣原体IgG滴度为阴性：结论：这对夫妇的不育可能是由于无症状和持续的慢性非淋菌性尿道炎相关感染引起的，而这种感染因后来再次感染非淋菌性尿道炎而变得复杂。这项研究表明，在不孕症病例调查中加入检测 nvCT 菌株的方法非常重要。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Open Microbiology Journal Immunology and Microbiology-Immunology and Microbiology (all)

CiteScore

1.80

自引率

0.00%

发文量

期刊介绍： The Open Microbiology Journal is a peer-reviewed open access journal which publishes research articles, reviews/mini-reviews, case studies, guest edited thematic issues and short communications/letters covering theoretical and practical aspects of Microbial systematics, evolutionary microbiology, immunology, virology, parasitology , bacteriology, mycology, phycology, protozoology, microbial ecology, molecular biology, microbial physiology, biochemistry, microbial pathogenesis, host-microbe interaction, systems microbiology, synthetic microbiology, bioinformatics. The Open Microbiology Journal , a peer-reviewed journal, is an important and reliable source of current information on developments in the field. The emphasis will be on publishing quality papers rapidly and freely available to researchers worldwide.