Human papillomavirus in head and neck squamous cell carcinomas in a South African cohort

IF 3.2

Papillomavirus Research Pub Date : 2018-12-01 DOI:10.1016/j.pvr.2018.10.006

Tumelo R. Sekee , Felicity J. Burt , Dominique Goedhals , Jacqueline Goedhals , Yuri Munsamy , Riaz Y. Seedat

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引用次数: 20

Abstract

Background

Most tumours of the head and neck are attributable to smoking and alcohol use, but an increasing proportion of head and neck tumours are caused by human papillomaviruses (HPVs). The aim of this study was to use in house molecular assays to detect and genotype HPV in biopsies from patients with histologically confirmed head and neck squamous cell carcinomas. In addition, the results were compared with p16 immunohistochemistry staining, which has been described as a potential marker for HPV infection.

Methods

Biopsies of squamous cell carcinomas of the oropharynx, nasopharynx, larynx and hypopharynx from 112 South African patients were screened using three PCR assays targeting the L1 and E6 regions of HPV and p16 immunohistochemical staining.

Results and conclusion

HPV was identified in 7 (6.3%) tumours, while 22 (19.6%) had positive p16 immunohistochemical staining. There was concordance between the results obtained using the three PCR assays. There was substantial agreement between the results of molecular tests and p16 immunohistochemistry for hypopharyngeal carcinomas, but only fair agreement for laryngeal and oropharyngeal carcinomas.

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南非人群头颈部鳞状细胞癌中的人乳头瘤病毒

大多数头颈部肿瘤可归因于吸烟和饮酒，但越来越多的头颈部肿瘤是由人乳头瘤病毒(hpv)引起的。本研究的目的是利用室内分子测定法检测组织学证实的头颈部鳞状细胞癌患者活检中的HPV并进行基因分型。此外，将结果与p16免疫组织化学染色进行比较，p16免疫组织化学染色被认为是HPV感染的潜在标志物。方法对112例南非患者的口咽、鼻咽、喉部和下咽鳞状细胞癌活检组织进行HPV L1和E6区和p16免疫组化染色的三种PCR检测。结果与结论7例(6.3%)肿瘤检出hpv, 22例(19.6%)肿瘤p16免疫组化染色阳性。用三种PCR方法得到的结果是一致的。下咽癌的分子检测和p16免疫组化结果基本一致，但喉癌和口咽癌的结果基本一致。

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来源期刊

Papillomavirus Research VIROLOGY-

自引率

0.00%

发文量

审稿时长

14 weeks

期刊介绍： The official Journal of the International Papillomavirus Society Papillomavirus Research (PVR), the Journal of HPV and other Small DNA Tumor Viruses publishes innovative papers related to all aspects of papillomaviruses and other small DNA tumor viruses. The official journal of the International Papillomavirus Society, PVR is an open access publication that aims to bring together virologists, immunologists, epidemiologists and clinicians working in the booming field of HPV and animal papillomaviruses, polyomaviruses and other small DNA tumor viruses and their associated diseases, in order to foster and facilitate interdisciplinary communication. The journal welcomes original research articles, reviews, short communications, opinion articles and regional update reports on papillomaviruses and other tumor viruses in the following sections: a. Biology of papillomaviruses and related viruses from life cycle to cancer b. Epidemiology etiology and natural history studies c. Natural and induced immunity including vaccine research d. Intervention studies and strategies including i. Clinical studies and trials ii. HPV treatments iii. HPV vaccination programs iv. Diagnostics and screening e. Infection and disease prevention, modeling studies f. Guidelines and public health recommendations g. HPV Studies in special populations Regional and local studies on these viruses.