Transformation of Recalcitrant Sorghum Varieties Facilitated by Baby Boom and Wuschel2

Q1 Agricultural and Biological Sciences

Current protocols in plant biology Pub Date : 2018-10-17 DOI:10.1002/cppb.20076

Kimberly Nelson-Vasilchik, Joel Hague, Muruganantham Mookkan, Zhanyuan J. Zhang, Albert Kausch

引用次数: 27

Abstract

Most reliable transformation protocols for cereal crops, including sorghum (Sorghum bicolor L. Moench), rely on the use of immature embryo explants to generate embryogenic callus cells that are then transformed using Agrobacterium- or particle-bombardment-mediated DNA delivery. Subsequent to DNA transfer, most protocols rely on selectable markers for the recovery of stably transformed callus that is then regenerated to produce T₀ plants. However, these protocols require specific genotypes that are innately capable of efficient embryogenic callus initiation. Here, we describe a system that makes use of the differential expression of the morphogenic regulators Baby Boom (Bbm) and Wuschel2 (Wus2) to achieve transformation in varieties of sorghum typically recalcitrant to standard transformation methods. © 2018 by John Wiley & Sons, Inc.

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“生育高峰”和“乌舍尔”促进了高粱品种的转化

最可靠的谷类作物转化方案，包括高粱(sorghum bicolor L. Moench)，依赖于使用未成熟胚胎外植体产生胚性愈伤组织细胞，然后使用农杆菌或颗粒轰击介导的DNA传递进行转化。在DNA转移之后，大多数方案依赖于可选择的标记来恢复稳定转化的愈伤组织，然后再生产生T0植株。然而，这些方案需要特定的基因型，天生能够有效的胚性愈伤组织起始。在这里，我们描述了一个系统，利用形态发生调控因子Baby Boom (Bbm)和Wuschel2 (Wus2)的差异表达，在标准转化方法通常难以实现的高粱品种中实现转化。©2018 by John Wiley &儿子,Inc。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current protocols in plant biology Agricultural and Biological Sciences-Plant Science

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期刊介绍： Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet nuances that are critical for an experiment''s success are not captured in the primary literature but exist only as part of a lab''s oral tradition. Current Protocols in Plant Biology provides reproducible step-by-step instructions for protocols relevant to plant research. Furthermore, Current Protocols content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly issues allow Current Protocols in Plant Biology to constantly evolve to keep pace with the newest discoveries and developments. Current Protocols in Plant Biology is the comprehensive source for protocols in the multidisciplinary field of plant biology, providing an extensive range of protocols from basic to cutting edge. Coverage includes: Extraction and analysis of DNA, RNA, proteins Chromosome analysis Transcriptional analysis Protein expression Metabolites Plant enzymology Epigenetics Plant genetic transformation Mutagenesis Arabidopsis, Maize, Poplar, Rice, and Soybean, and more.