Evidence of BVDV in Pigs from North Eastern Part of India- Genetic Profiling and Characterisation.

The Open Virology Journal Pub Date : 2018-08-31 eCollection Date: 2018-01-01 DOI:10.2174/1874357901812010110
Amit Kr Chakraborty, Priyanka Mukherjee, Amarjit Karam, Samir Das, Luit Barkalita, Kekungo Puro, Rajkumari Sanjukta, Sandeep Ghatak, Ingudam Sakuntala, Ram Gopal Laha, Prabodh Borah, S V Ngachan, Indu Sharma, Arnab Sen
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引用次数: 4

Abstract

Introduction: The work has been attempted to detect and genetically characterise the nature of Bovine Viral Diarrhea Virus (BVDV) isolates from the porcine population of the north east.

Methods and material: The samples have been collected over a two year period and are from areas where there is a mixed and integrated rearing of livestock in close proximity. The isolates were identified, cloned and sequenced using BVD specific genomic primers for two important domains viz., E-2 and 5' UTR.

Results: Porcine BVD Sequences were analysed phylogenetically. Divergence in 3 sequences is noted in the 5' UTR region that are forming a clear outlier group while E-2 sequences are coming close to BVDV group but forming a separate cluster.

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印度东北部猪BVDV的证据-遗传谱和特征。
本研究旨在检测从东北猪群中分离出的牛病毒性腹泻病毒(BVDV)的性质并对其进行遗传表征。方法和材料:样本是在两年期间收集的,并来自附近有混合和综合饲养牲畜的地区。利用BVD特异性基因组引物对两个重要结构域(E-2和5' UTR)进行鉴定、克隆和测序。结果:对猪BVD序列进行了系统发育分析。3个序列在5' UTR区出现分化,形成明显的离群,而E-2序列与BVDV组接近,但形成单独的簇。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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