MicroRNA expression profile of urinary exosomes in Type IV lupus nephritis complicated by cellular crescent.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2018-10-04 eCollection Date: 2018-12-01 DOI:10.1186/s40709-018-0088-0

Yi Li, Xiaosong Xu, Xiaopeng Tang, Xiuwu Bian, Bingbing Shen, Hongwen Zhao, Shiyuan Luo, Zhiwen Chen, Keqin Zhang

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引用次数: 28

Abstract

Background: Type IV lupus nephritis (LNIV) is a severe disease characterized by diffuse proliferative lesions, and its prognosis is worse with cellular crescent (LNIV-CC) involvement. Urinary exosomes have been shown to reflect the degree of kidney injury. This study was aimed to identify non-invasive diagnostic markers for LNIV-CC. We analysed the expression profile of microRNAs (miRNAs) isolated from urinary exosomes in patients with LNIV-CC and LNIV, and healthy individuals using high-throughput sequencing.

Results: A total of 66 differentially expressed miRNAs were identified, which were significantly enriched in 15 signalling pathways. Bioinformatic analysis revealed a co-expression network of miRNAs, predicted transcription factors and target mRNAs. Expression of three miRNAs including miR-3135b, miR-654-5p, and miR-146a-5p were further analysed and validated by reverse transcription-quantitative polymerase chain reaction. ROC analysis suggested these as candidate biomarkers for LNIV-CC.

Conclusions: LNIV-CC has a unique miRNA expression profile of urinary exosome and complex regulatory network. miR-3135b, miR-654-5p and miR-146a-5p in urinary exosomes could be used as novel non-invasive diagnostic markers for LNIV-CC.

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IV型狼疮性肾炎合并细胞月牙的尿外泌体MicroRNA表达谱。

背景:IV型狼疮性肾炎(LNIV)是一种以弥漫性增生性病变为特征的重症疾病，当细胞新月(LNIV- cc)受累时，其预后较差。尿外泌体已被证明可以反映肾脏损伤的程度。本研究旨在确定LNIV-CC的非侵入性诊断标志物。我们利用高通量测序技术分析了从LNIV- cc和LNIV患者以及健康个体的尿外泌体中分离的microRNAs (miRNAs)的表达谱。结果:共鉴定出66个差异表达的mirna，它们在15条信号通路中显著富集。生物信息学分析揭示了mirna的共表达网络，预测了转录因子和靶mrna。通过逆转录-定量聚合酶链反应进一步分析和验证miR-3135b、miR-654-5p和miR-146a-5p三种mirna的表达。ROC分析表明这些是LNIV-CC的候选生物标志物。结论:LNIV-CC具有独特的尿外泌体miRNA表达谱和复杂的调控网络。尿外泌体中的miR-3135b、miR-654-5p和miR-146a-5p可作为LNIV-CC的新型无创诊断标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.