MicroRNA expression profile of urinary exosomes in Type IV lupus nephritis complicated by cellular crescent.

Pub Date : 2018-10-04 eCollection Date: 2018-12-01 DOI:10.1186/s40709-018-0088-0

Yi Li, Xiaosong Xu, Xiaopeng Tang, Xiuwu Bian, Bingbing Shen, Hongwen Zhao, Shiyuan Luo, Zhiwen Chen, Keqin Zhang

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引用次数: 28

Abstract

Background: Type IV lupus nephritis (LNIV) is a severe disease characterized by diffuse proliferative lesions, and its prognosis is worse with cellular crescent (LNIV-CC) involvement. Urinary exosomes have been shown to reflect the degree of kidney injury. This study was aimed to identify non-invasive diagnostic markers for LNIV-CC. We analysed the expression profile of microRNAs (miRNAs) isolated from urinary exosomes in patients with LNIV-CC and LNIV, and healthy individuals using high-throughput sequencing.

Results: A total of 66 differentially expressed miRNAs were identified, which were significantly enriched in 15 signalling pathways. Bioinformatic analysis revealed a co-expression network of miRNAs, predicted transcription factors and target mRNAs. Expression of three miRNAs including miR-3135b, miR-654-5p, and miR-146a-5p were further analysed and validated by reverse transcription-quantitative polymerase chain reaction. ROC analysis suggested these as candidate biomarkers for LNIV-CC.

Conclusions: LNIV-CC has a unique miRNA expression profile of urinary exosome and complex regulatory network. miR-3135b, miR-654-5p and miR-146a-5p in urinary exosomes could be used as novel non-invasive diagnostic markers for LNIV-CC.

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IV型狼疮性肾炎合并细胞月牙的尿外泌体MicroRNA表达谱。

背景:IV型狼疮性肾炎(LNIV)是一种以弥漫性增生性病变为特征的重症疾病，当细胞新月(LNIV- cc)受累时，其预后较差。尿外泌体已被证明可以反映肾脏损伤的程度。本研究旨在确定LNIV-CC的非侵入性诊断标志物。我们利用高通量测序技术分析了从LNIV- cc和LNIV患者以及健康个体的尿外泌体中分离的microRNAs (miRNAs)的表达谱。结果:共鉴定出66个差异表达的mirna，它们在15条信号通路中显著富集。生物信息学分析揭示了mirna的共表达网络，预测了转录因子和靶mrna。通过逆转录-定量聚合酶链反应进一步分析和验证miR-3135b、miR-654-5p和miR-146a-5p三种mirna的表达。ROC分析表明这些是LNIV-CC的候选生物标志物。结论:LNIV-CC具有独特的尿外泌体miRNA表达谱和复杂的调控网络。尿外泌体中的miR-3135b、miR-654-5p和miR-146a-5p可作为LNIV-CC的新型无创诊断标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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