Samantha Solito, Laura Pinton, Francesco De Sanctis, Stefano Ugel, Vincenzo Bronte, Susanna Mandruzzato, Ilaria Marigo
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引用次数: 32
Abstract
This unit presents methods to assess the immunosuppressive properties of immunoregulatory cells of myeloid origin, such as myeloid-derived suppressor cells (MDSCs), both in vitro and in vivo in mice, as well as in biological samples from cancer patients. These methods could be adapted to test the impact of different suppressive populations on T cell activation, proliferation, and cytotoxic activity; moreover, they could be useful to assess the influence exerted by genetic modifications, chemical inhibitors, and drugs on immune suppressive pathways © 2018 by John Wiley & Sons, Inc.
方法测定MDSC体外和体内免疫抑制活性
本单元介绍了评估骨髓源性免疫调节细胞(如骨髓源性抑制细胞(MDSCs))的免疫抑制特性的方法，包括体外和体内小鼠，以及来自癌症患者的生物样本。这些方法可用于测试不同抑制群体对T细胞活化、增殖和细胞毒性活性的影响;此外，它们还可用于评估基因修饰、化学抑制剂和药物对免疫抑制途径的影响©2018 by John Wiley &儿子,Inc。
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