Generation of Stable Transgenic Rice (Oryza sativa L.) by Agrobacterium-Mediated Transformation

Q1 Agricultural and Biological Sciences

Current protocols in plant biology Pub Date : 2016-06-10 DOI:10.1002/cppb.20004

Yi Zhang, Jun Li, Caixia Gao

引用次数: 8

Abstract

Rice (Oryza sativa L.) is the staple food for more than half of the world's population, and has also become an important model monocot. As a result, numerous genetic transformation protocols have been developed to improve and better understand this particular agronomic plant. Here we introduce a convenient transformation method using Agrobacterium. The explants used are embryogenic calli derived from mature seeds, which are easily obtained and can be used all year round. After selection and regeneration, transformants are obtained from resistant calli cultured on the regeneration medium. This protocol has been used to generate transgenic rice (Oryza sativa L.) in as little as 4 months. © 2016 by John Wiley & Sons, Inc.

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农杆菌介导转化制备稳定转基因水稻

水稻(Oryza sativa L.)是世界上一半以上人口的主食，也已成为重要的单子叶作物模式。因此，已经开发了许多遗传转化方案来改进和更好地了解这种特殊的农艺植物。本文介绍了一种利用农杆菌进行转化的简便方法。所采用的外植体为成熟种子的胚性愈伤组织，易获得，一年四季均可使用。抗性愈伤组织经筛选和再生后，在再生培养基上培养获得转化体。该方案已用于在短短4个月内产生转基因水稻(Oryza sativa L.)。©2016 by John Wiley &儿子,Inc。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current protocols in plant biology Agricultural and Biological Sciences-Plant Science

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期刊介绍： Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet nuances that are critical for an experiment''s success are not captured in the primary literature but exist only as part of a lab''s oral tradition. Current Protocols in Plant Biology provides reproducible step-by-step instructions for protocols relevant to plant research. Furthermore, Current Protocols content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly issues allow Current Protocols in Plant Biology to constantly evolve to keep pace with the newest discoveries and developments. Current Protocols in Plant Biology is the comprehensive source for protocols in the multidisciplinary field of plant biology, providing an extensive range of protocols from basic to cutting edge. Coverage includes: Extraction and analysis of DNA, RNA, proteins Chromosome analysis Transcriptional analysis Protein expression Metabolites Plant enzymology Epigenetics Plant genetic transformation Mutagenesis Arabidopsis, Maize, Poplar, Rice, and Soybean, and more.