{"title":"POTENTIATION EFFECTS OF 3-AMINOBENZAMIDE ON DIVALENT CATION-DEPENDENT DNA FRAGMENTATION IN MAMMALIAN CELLS EXPOSED TO METHYL METHANESULFONATE.","authors":"J K Park, I S Park, J S Lee, S D Park","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>THE PROPERTIES OF METHYL METHANESULFONATE (MMS) DAMAGE-RELATED NUCLEASE ACTIVITY IN CHO AND HELA CELLS WERE STUDIED BY NEUTRAL AND ALKALINE ELUTION TECHNIQUES. THE FRAGMENTATION OF MMS-TREATED DNA WAS DEPENDENT ON THE PRESENCE OF CA++ AND MG++ IONS, AND WAS NOT OBSERVED UPON TREATMENT WITH DIVALENT ION CHELATORS SUCH AS EDTA OR EGTA. THE CA++ AND MG++ -DEPENDENT CONVERSION OF MMS-TREATED SUPERCOILED PLASMID PREVIOUSLY REACTED WITH CHO CELL NUCLEAR EXTRACT TO OPEN CIRCULAR FORM WAS MARKEDLY INCREASED IN A 3-AMINOBENZAMIDE (3-AB) DOSE-DEPENDENT MANNER AS DETERMINED BY AGAROSE GEL ELECTROPHORESIS AND DENSITOMETRIC ANALYSIS. THESE RESULTS SUGGEST THAT THE INHIBITION OF POLY (ADP-RIBOSE) POLYMERASE BY 3-AB RESULTS IN THE ACTIVATION OF THE CA++, MG++ -DEPENDENT ENDONUCLEASE WHICH NONSPECIFICALLY CUTS MMS-TREATED DNA.</p>","PeriodicalId":89152,"journal":{"name":"In vitro toxicology","volume":"4 1","pages":"73-9"},"PeriodicalIF":0.0000,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"In vitro toxicology","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
THE PROPERTIES OF METHYL METHANESULFONATE (MMS) DAMAGE-RELATED NUCLEASE ACTIVITY IN CHO AND HELA CELLS WERE STUDIED BY NEUTRAL AND ALKALINE ELUTION TECHNIQUES. THE FRAGMENTATION OF MMS-TREATED DNA WAS DEPENDENT ON THE PRESENCE OF CA++ AND MG++ IONS, AND WAS NOT OBSERVED UPON TREATMENT WITH DIVALENT ION CHELATORS SUCH AS EDTA OR EGTA. THE CA++ AND MG++ -DEPENDENT CONVERSION OF MMS-TREATED SUPERCOILED PLASMID PREVIOUSLY REACTED WITH CHO CELL NUCLEAR EXTRACT TO OPEN CIRCULAR FORM WAS MARKEDLY INCREASED IN A 3-AMINOBENZAMIDE (3-AB) DOSE-DEPENDENT MANNER AS DETERMINED BY AGAROSE GEL ELECTROPHORESIS AND DENSITOMETRIC ANALYSIS. THESE RESULTS SUGGEST THAT THE INHIBITION OF POLY (ADP-RIBOSE) POLYMERASE BY 3-AB RESULTS IN THE ACTIVATION OF THE CA++, MG++ -DEPENDENT ENDONUCLEASE WHICH NONSPECIFICALLY CUTS MMS-TREATED DNA.
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