INDUCTION OF UNSTABLE MUTATIONS IN DROSOPHILA MELANOGASTER BY THE MICROINJECTION OF ONCOGENIC VIRAL DNA INTO THE POLAR PLASM OF EMBRYOS: PROLONGED GENETIC INSTABILITY OF MUTATIONS AT THE LOBE LOCUS.

Abstract

MUTATIONS IN THE LOBE LOCUS WERE PRODUCED BY MICROINJECTION OF RSV CDNA INTO EARLY EMBRYOS OF D. MELANOGASTER, AND SHOWED PROLONGED GENETIC INSTABILITY, THE EXTENT OF WHICH CHANGED SIGNIFICANTLY WITH TIME. GENETIC ANALYSIS OF LOBE-RSV MUTATIONS AND A MOLECULAR-BIOLOGICAL ANALYSIS OF STRUCTURAL REARRANGEMENTS IN THE W AND ADH LOCI, WHICH ARE FREQUENTLY MUTATED BY RETROVIRAL CDNA, SHOWED THAT THE MUTATIONS RESULTED FROM INSERTIONS ARISING IN THIS GENETIC INSTABILITY SYSTEM.