Effects of Aged Oil Sludge on Soil Physicochemical Properties and Fungal Diversity Revealed by High-Throughput Sequencing Analysis.

IF 2.3 4区生物学 Q3 MICROBIOLOGY

Archaea-An International Microbiological Journal Pub Date : 2018-09-06 eCollection Date: 2018-01-01 DOI:10.1155/2018/9264259

Huihui Wang, Shaoping Kuang, Qiaolin Lang, Wenjuan Yu

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引用次数: 13

Abstract

The oilfield soil was contaminated for years by large quantities of aged oil sludge generated in the petroleum industry. In this study, physicochemical properties, contents of main pollutants, and fungal diversity of the aged oil sludge-contaminated soil were analyzed. Results revealed that aged oil sludge significantly changed physical and chemical properties of the receiving soil and increased the contents of main pollutants (petroleum hydrocarbons and heavy metals) in soil. Meanwhile, the internal transcribed spacer (ITS) sequencing by Illumina Miseq platform at each taxonomic level demonstrated that the toxicological effect of oil pollutants obviously influenced the fungal diversity and community structure in soil. Moreover, it was found that the presence of three genera (Cephalotheca, Lecanicillium, and Septoriella) appeared in aged oil sludge-contaminated soil. And oil pollutants promoted the growth of certain genera in Ascomycota (70.83%) and Basidiomycota (10.78%), such as Venturia, Alternaria, and Piloderma. Nevertheless, the growth of Mortierella (9.16%), Emericella (6.02%), and Bjerkandera (0.00%) was intensively limited. This study would aid thorough understanding of microbial diversity in oil-contaminated soil and thus provide new point of view to soil bioremediation.

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老化油泥对土壤理化性质及真菌多样性的影响

多年来，油田土壤受到石油工业中产生的大量老化油泥的污染。对油泥污染土壤的理化性质、主要污染物含量及真菌多样性进行了分析。结果表明，老化的油泥显著改变了接收土壤的理化性质，增加了土壤中主要污染物(石油烃和重金属)的含量。同时，利用Illumina Miseq平台在各个分类水平上对其内部转录间隔序列(ITS)进行测序，结果表明石油污染物的毒理学效应对土壤真菌多样性和群落结构有明显影响。此外，在陈旧的油泥污染土壤中发现了3个属(Cephalotheca, Lecanicillium, Septoriella)的存在。油类污染物对子囊菌门(70.83%)和担子菌门(10.78%)中Venturia、Alternaria和Piloderma等属的生长有促进作用。然而，Mortierella(9.16%)、Emericella(6.02%)和Bjerkandera(0.00%)的生长受到强烈限制。本研究有助于深入了解石油污染土壤微生物多样性，为土壤生物修复提供新的思路。

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来源期刊

Archaea-An International Microbiological Journal MICROBIOLOGY-

CiteScore

7.50

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Archaea is a peer-reviewed, Open Access journal that publishes original research articles as well as review articles dealing with all aspects of archaea, including environmental adaptation, enzymology, genetics and genomics, metabolism, molecular biology, molecular ecology, phylogeny, and ultrastructure. Bioinformatics studies and biotechnological implications of archaea will be considered. Published since 2002, Archaea provides a unique venue for exchanging information about these extraordinary prokaryotes.