Evaluation of different commercial hyaluronic acids as a vehicle for injection of human adipose-derived mesenchymal stem cells

Revista Brasileira de Ortopedia (English Edition) Pub Date : 2018-09-01 DOI:10.1016/j.rboe.2018.07.009

Camila Cohen Kaleka , Eder Zucconi , Tierri da Silva Vieira , Mariane Secco , Mário Ferretti , Moisés Cohen

{"title":"Evaluation of different commercial hyaluronic acids as a vehicle for injection of human adipose-derived mesenchymal stem cells","authors":"Camila Cohen Kaleka , Eder Zucconi , Tierri da Silva Vieira , Mariane Secco , Mário Ferretti , Moisés Cohen","doi":"10.1016/j.rboe.2018.07.009","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><p>The main purpose of this study is to evaluate, <em>in vitro</em>, the cytotoxicity of different commercial brands of hyaluronic acids to be used as a vehicle for injection of human adipose-derived mesenchymal stem cells (AD-MSCs).</p></div><div><h3>Methods</h3><p>AD-MSCs were divided into seven groups: one control group where AD-MSCs were cultivated with phosphate-buffered saline (PBS) and six other groups where AD-MSCs were cultivated with different commercial brands of hyaluronic acid. AD-MSC viability analysis was performed after 4, 24, and 48h in contact with each treatment, using the trypan staining method on a Countess automated cell counter (Thermo Fisher Scientific).</p></div><div><h3>Results</h3><p>The results clearly demonstrated a significant difference in cell viability when AD-MSCs were exposed to different hyaluronic acids when compared with the control group.</p></div><div><h3>Conclusion</h3><p>These data suggest that hyaluronic acid can be used as a vehicle for injection of human AD-MSCs, but caution is needed to choose the best product, aiming at its future therapeutic application.</p></div>","PeriodicalId":101095,"journal":{"name":"Revista Brasileira de Ortopedia (English Edition)","volume":"53 5","pages":"Pages 557-563"},"PeriodicalIF":0.0000,"publicationDate":"2018-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.rboe.2018.07.009","citationCount":"6","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Revista Brasileira de Ortopedia (English Edition)","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2255497118300983","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 6

Abstract

Objective

The main purpose of this study is to evaluate, in vitro, the cytotoxicity of different commercial brands of hyaluronic acids to be used as a vehicle for injection of human adipose-derived mesenchymal stem cells (AD-MSCs).

Methods

AD-MSCs were divided into seven groups: one control group where AD-MSCs were cultivated with phosphate-buffered saline (PBS) and six other groups where AD-MSCs were cultivated with different commercial brands of hyaluronic acid. AD-MSC viability analysis was performed after 4, 24, and 48 h in contact with each treatment, using the trypan staining method on a Countess automated cell counter (Thermo Fisher Scientific).

Results

The results clearly demonstrated a significant difference in cell viability when AD-MSCs were exposed to different hyaluronic acids when compared with the control group.

Conclusion

These data suggest that hyaluronic acid can be used as a vehicle for injection of human AD-MSCs, but caution is needed to choose the best product, aiming at its future therapeutic application.

Abstract Image

查看原文本刊更多论文

不同商用透明质酸作为人脂肪源间充质干细胞注射载体的评价

目的本研究的主要目的是在体外评估不同商业品牌的透明质酸作为人脂肪源性间充质干细胞(AD-MSCs)注射载体的细胞毒性。方法将AD-MSCs分为7组:1组采用磷酸盐缓冲盐水(PBS)培养AD-MSCs, 6组采用不同品牌透明质酸培养AD-MSCs。在伯爵夫人自动细胞计数器(Thermo Fisher Scientific)上使用台锥虫染色法，在每种处理接触4、24和48小时后进行AD-MSC活力分析。结果AD-MSCs暴露于不同透明质酸后，细胞活力与对照组相比有显著差异。结论透明质酸可作为人AD-MSCs的注射载体，但针对其未来的治疗应用，需谨慎选择最佳产品。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Revista Brasileira de Ortopedia (English Edition)

自引率

0.00%

发文量