ERCC5 rs751402 polymorphism is the risk factor for sporadic breast cancer in Thailand.

Abstract

Breast cancer is a complex disease. Single Nucleotide Polymorphisms (SNPs) can modify the risk of cancer. They may be regarded as potential markers of carcinogenesis. Currently, the diversity or polymorphism of ERCC5 gene (excision repair cross-complementary group 5 gene or ERCC5) was reported to associate with an increased risk of breast cancer. This study aims to investigate the relationship between ERCC5 polymorphism and the breast cancer risk in the lower northeastern region women of Thailand. One hundred fifty five samples from breast cancer patients and 122 samples from healthy control group were analysed. Genomic DNA was extracted from white blood cell of all samples. The real-time polymerase chain reaction (qPCR) was used to demonstrate genetic polymorphism of ERCC5. The results showed that the ERCC5 rs751402 polymorphism variant AG was associated with an increased risk of breast cancer. The frequency of ERCC5 rs751402 in patients with breast cancer was higher than healthy control group. The ERCC5 rs751402 variant AG carrier was associated with increased breast cancer risk to 2.3 folds, with OR = 2.30, 95% CI = 1.22-4.35, P = 0.01, when age, menopause period, number of child, smoking and alcohol drinking were adjust. This study demonstrated that ERCC5 rs751402 genotype AG was associated with breast cancer risk in the lower northeastern region women of Thailand.