Structural and Functional Analysis of the Gut Microbiome for Toxicologists

Robert G. Nichols, Jingwei Cai, Iain A. Murray, Imhoi Koo, Philip B. Smith, Gary H. Perdew, Andrew D. Patterson
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引用次数: 5

Abstract

Characterizing the reciprocal interactions between toxicants, the gut microbiota, and the host, holds great promise for improving our mechanistic understanding of toxic endpoints. Advances in culture-independent sequencing analysis (e.g., 16S rRNA gene amplicon sequencing) combined with quantitative metabolite profiling (i.e., metabolomics) have provided new ways of studying the gut microbiome and have begun to illuminate how toxicants influence the structure and function of the gut microbiome. Developing a standardized protocol is important for establishing robust, reproducible, and importantly, comparative data. This protocol can be used as a foundation for examining the gut microbiome via sequencing-based analysis and metabolomics. Two main units follow: (1) analysis of the gut microbiome via sequencing-based approaches; and (2) functional analysis of the gut microbiome via metabolomics. © 2018 by John Wiley & Sons, Inc.

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毒理学家肠道微生物组的结构和功能分析
表征毒素、肠道微生物群和宿主之间的相互作用,对提高我们对毒性终点的机制理解有很大的希望。与培养无关的测序分析(如16S rRNA基因扩增子测序)结合定量代谢物谱分析(即代谢组学)的进展为研究肠道微生物组提供了新的方法,并开始阐明毒物如何影响肠道微生物组的结构和功能。制定标准化协议对于建立可靠的、可重复的、重要的、可比较的数据非常重要。该方案可作为通过基于测序的分析和代谢组学检查肠道微生物组的基础。以下两个主要单元:(1)通过基于测序的方法分析肠道微生物组;(2)通过代谢组学分析肠道微生物组的功能。©2018 by John Wiley &儿子,Inc。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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