[Study on Total Triterpenoids Content of Enrichment Mineral Elements of Inonotus Obliquus].

中药材 Pub Date : 2016-08-01

Yan Yu, Xiao-fan Guo, Yu-feng Wang, Liang Wang, Bing-Yan Liu

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引用次数: 0

Abstract

Objective: To study the influence of different mineral elements enrichment of calcium, iron, and zinc on the growth of the mycelium from Inonotus obliquus, to explore the influence on total triterpenoids content of Inonotus obliquus.

Methods: Mineral elements were enriched on Inonotus obliquus, total triterpenoids extraction methods and determination methods were optimized, and total triterpenoids content of Inonotus obliquus were compared through different mineral elements enrichment.

Results: The mycelial growth and yield were promoted obviously when 1 000 mg/L Ca(NO3)2 was added in the culture medium, and the calcium enrichment rate could reach to 0.07%. The most appropriate iron source was Fe2(SO4)3, and the iron enrichment rate could reach to 0.29% when 300 mg/L Fe2(SO4)3 was added. 175 mg/L Zn SO4 led to the strongest mycelium growth vigor, and the zinc enrichment rate could reach to 0.57%. The total triterpenoids content of Inonotus obliquus was the highest (0.058 mg/mL) as enrichment of calcium element, the total triterpenoids content of Inonotus obliquus was the least (0.04 mg/mL) as enrichment of iron elements.

Conclusion: The total triterpenoids content of Inonotus obliquus was the highest when calcium element was enriched. This study provides a new method and basic data for the factory production of Inonotus obliquus.

本刊更多论文

[牛腹草富集矿物元素中总三萜含量的研究]。

目的:研究不同矿质元素富集钙、铁、锌对斜边野菇菌丝体生长的影响，探讨其对斜边野菇总三萜含量的影响。方法:对斜柳中矿物元素进行富集，优化总三萜的提取方法和测定方法，通过不同矿物元素富集程度对斜柳中总三萜的含量进行比较。结果:当培养基中添加1 000 mg/L Ca(NO3)2时，菌丝生长和产量得到明显促进，钙富集率可达0.07%。最适宜的铁源为Fe2(SO4)3，当添加300 mg/L Fe2(SO4)3时，铁富集率可达0.29%。175 mg/L Zn SO4处理菌丝生长活力最强，锌富集率可达0.57%。对钙元素的富集，斜叶鹭的总三萜含量最高(0.058 mg/mL);对铁元素的富集，斜叶鹭的总三萜含量最低(0.04 mg/mL)。结论:在钙元素富集的条件下，斜柳总三萜含量最高。本研究为斜叶莲的工厂化生产提供了新的方法和基础资料。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

中药材

自引率

0.00%

发文量

11364

期刊介绍： "Chinese Materia Medica" magazine was founded in 1978. It is a national-level academic journal of Chinese medicine science and technology approved by the State Science and Technology Commission and the Press and Publication Administration for public distribution at home and abroad. It is sponsored by the Chinese Materia Medica Information Center of the State Food and Drug Administration and is publicly distributed at home and abroad. It mainly reports on new technologies, new methods and new achievements in the production, scientific research and business management of Chinese medicine, and serves to promote the production and scientific and technological development of Chinese medicine, improve the output and quality of Chinese medicine, improve business management, and prosper the Chinese medicine industry.