[AFLP and HPLC Fingerprints Analysis of Codonopsis Species from Original Areas and the Same Planting Base].

中药材 Pub Date : 2016-08-01

Cong Gu, Ling-ya Cao, Qiang Su, Lin-jing Guan, Jing Yang, Jian-ping Gao

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引用次数: 0

Abstract

Objective: To investigate the influence of environment on the genetic structure and chemical ingredients of different Codonopsis species.

Methods: Amplified fragment length polymorphism( AFLP) molecular markers were used to analyze the genetic polymorphism of Codonopsis species from 24 different populations,and high performance liquid chromatography( HPLC) fingerprints were performed to analyze the chemical ingredients of 10 batches of Codonopsis roots.

Results: According to species classification,the 24 populations were categorized into three groups by UPGMA cluster method. The samples of Codonopsis pilosula var. modesta introduced from Wenxian( Gansu Province) and Jiuzhaigou County( Sichuan Province) were clustered into Group Ⅰ. The Codonopsis tangshen population introduced from Banqiao( Hubei Province) was clustered into Group Ⅱ. The populations from Codonopsis pilosula introduced to Shanxi Province GAP base,and the wild and cultured populations of Codonopsis pilosula collected from Shanxi Province were into Group Ⅲ. The similarity of HPLC fingerprints among three Codonopsis species introduced into the same environment was over 0. 8.

Conclusion: Genetic differences of three Codonopsis species from original areas and Codonopsis species introduced into Shanxi Province were caused by their inter-species genetic characteristic. Meanwhile, the similarities of genetic backgrounds in Codonopsis species were related to geographic space, and chemical ingredients of Codonopsis were easily influenced by the cultivation environment.

本刊更多论文

[产地和同一种植基地党参种类的AFLP和HPLC指纹图谱分析]。

目的:探讨不同环境对党参遗传结构和化学成分的影响。方法:采用扩增片段长度多态性(AFLP)分子标记分析24个不同群体党参种的遗传多态性，并采用高效液相色谱(HPLC)指纹图谱对10批党参根的化学成分进行分析。结果:采用UPGMA聚类法将24个种群按物种分类分为3类。从甘肃温县和四川九寨沟县引进的参党参样本聚为Ⅰ群。从湖北板桥引种的唐参居群聚为Ⅱ群。引种到山西GAP基地的党参种群和从山西采集到的党参野生种群和养殖种群归为Ⅲ组。引入同一环境的三种党参HPLC指纹图谱相似度均大于0。8.结论:原属地三种党参与山西省引种党参的遗传差异是由其种间遗传特征引起的。同时，党参物种遗传背景的相似性与地理空间有关，党参化学成分易受栽培环境的影响。

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来源期刊

中药材

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发文量

11364

期刊介绍： "Chinese Materia Medica" magazine was founded in 1978. It is a national-level academic journal of Chinese medicine science and technology approved by the State Science and Technology Commission and the Press and Publication Administration for public distribution at home and abroad. It is sponsored by the Chinese Materia Medica Information Center of the State Food and Drug Administration and is publicly distributed at home and abroad. It mainly reports on new technologies, new methods and new achievements in the production, scientific research and business management of Chinese medicine, and serves to promote the production and scientific and technological development of Chinese medicine, improve the output and quality of Chinese medicine, improve business management, and prosper the Chinese medicine industry.