[Study of Berberine on Attenuating PM2.5-Induced Vascular Endothelial Cells Injury by ERK1/2 Signal Pathway].

中药材 Pub Date : 2016-07-01

Qiang Wan, Yu-ping Yang, Zhong-yong Liu

{"title":"[Study of Berberine on Attenuating PM2.5-Induced Vascular Endothelial Cells Injury by ERK1/2 Signal Pathway].","authors":"Qiang Wan, Yu-ping Yang, Zhong-yong Liu","doi":"","DOIUrl":null,"url":null,"abstract":"Objective: To investigate the effect and mechanism of berberine in attenuating PM2. 5-induced human umbilical vein endothelial cells EA. hy926 injury.Methods: The samples of fine particulate matter( PM2. 5) were collected and made into suspension. Different concentrations of PM2. 5( 0,20,200,400 mg / L) were added into EA. hy926 cells for 24 h. The viability of EA. hy926 cells was detected by MTT assay, and apoptosis of EA. hy926 cells was detected by flow cytometry, the expressions of p-ERK1 /2,BAX and BCL-2 in the EA. hy926 cells were measured by Western blot,the contents of IL-6,TNF-α were measured by ELISA, the content of MDA, and the activities of SOD and LDH in the EA. hy926 cells culture supernatant were measured, respectively. Different concentrations of berberine( 10,50,100 μmol / L) and PD98059( 20 μmol / L) was added into the EA. hy926 cells to observe the effect of berberine.Results: Compared with control group,PM2. 5 decreased the viability in a dose dependent manner, and significantly upgraded the protein levels of p-ERK1 /2 and BAX / BCL-2 ratio,PM2. 5 increased the apoptosis of EA. hy926 cells, and increased the contents of IL-6,TNF-α and MDA, increased the activity of LDH, and decreased SOD activity in the EA. hy926 cells( P < 0. 05). Compared with PM2. 5 group, berberine increased the viability of EA. hy926 cells in a dose dependent manner,PM2. 5 significantly downgraded the protein levels of p-ERK1 /2 and BAX / BCL-2 ratio, and decreased the apoptosis of EA. hy926 cells, decreased the contents of IL-6,TNF-α and MDA, and decreased the activity of LDH, and increased SOD activity in the EA. hy926 cells( P < 0. 05).Conclusion: Berberine attenuates PM2. 5-induced EA. hy926 cells injury by inhibiting ERK1 /2 pathway.","PeriodicalId":15312,"journal":{"name":"中药材","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"中药材","FirstCategoryId":"3","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

Abstract

Objective: To investigate the effect and mechanism of berberine in attenuating PM2. 5-induced human umbilical vein endothelial cells EA. hy926 injury.

Methods: The samples of fine particulate matter( PM2. 5) were collected and made into suspension. Different concentrations of PM2. 5( 0,20,200,400 mg / L) were added into EA. hy926 cells for 24 h. The viability of EA. hy926 cells was detected by MTT assay, and apoptosis of EA. hy926 cells was detected by flow cytometry, the expressions of p-ERK1 /2,BAX and BCL-2 in the EA. hy926 cells were measured by Western blot,the contents of IL-6,TNF-α were measured by ELISA, the content of MDA, and the activities of SOD and LDH in the EA. hy926 cells culture supernatant were measured, respectively. Different concentrations of berberine( 10,50,100 μmol / L) and PD98059( 20 μmol / L) was added into the EA. hy926 cells to observe the effect of berberine.

Results: Compared with control group,PM2. 5 decreased the viability in a dose dependent manner, and significantly upgraded the protein levels of p-ERK1 /2 and BAX / BCL-2 ratio,PM2. 5 increased the apoptosis of EA. hy926 cells, and increased the contents of IL-6,TNF-α and MDA, increased the activity of LDH, and decreased SOD activity in the EA. hy926 cells( P < 0. 05). Compared with PM2. 5 group, berberine increased the viability of EA. hy926 cells in a dose dependent manner,PM2. 5 significantly downgraded the protein levels of p-ERK1 /2 and BAX / BCL-2 ratio, and decreased the apoptosis of EA. hy926 cells, decreased the contents of IL-6,TNF-α and MDA, and decreased the activity of LDH, and increased SOD activity in the EA. hy926 cells( P < 0. 05).

Conclusion: Berberine attenuates PM2. 5-induced EA. hy926 cells injury by inhibiting ERK1 /2 pathway.

本刊更多论文

[小檗碱通过ERK1/2信号通路减弱pm2.5诱导的血管内皮细胞损伤的研究]。

目的:探讨小檗碱对PM2的抑制作用及其机制。5诱导人脐静脉内皮细胞EA. hy926损伤。方法:提取细颗粒物(PM2)样品。5)收集成悬浮液。不同浓度的PM2。5 (0, 20200400 mg / L)被添加到EA. hy926细胞24 h。的可行性EA. hy926细胞MTT试验,检测和细胞凋亡EA. hy926细胞流式细胞术,检测p-ERK1 / 2的表情,伯灵顿和bcl - 2在EA. hy926细胞被免疫印迹测量,il - 6的内容,TNF -α测量通过ELISA、MDA的含量和SOD的活动和LDH EA. hy926细胞文化浮在表面的测量,分别。在EA. hy926细胞中加入不同浓度的小檗碱(10、50、100 μmol / L)和PD98059(20 μmol / L)，观察小檗碱的作用。结果:与对照组比较，PM2。5 .细胞活力呈剂量依赖性降低，p-ERK1 /2和BAX / BCL-2比值PM2蛋白水平显著升高。5增加EA. hy926细胞凋亡，增加IL-6、TNF-α、MDA含量，提高LDH活性，降低SOD活性(P < 0.05)。05）. 与PM2比较。5组，小檗碱对EA. hy926细胞的存活率呈剂量依赖性，PM2。5显著降低EA. hy926细胞P - erk1 /2蛋白水平和BAX / BCL-2蛋白比值，减少EA. hy926细胞凋亡，降低IL-6、TNF-α和MDA含量，降低LDH活性，升高SOD活性(P < 0.05)。05)．结论:小檗碱对PM2有减弱作用。5通过抑制ERK1 /2通路诱导EA. hy926细胞损伤。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

中药材

自引率

0.00%

发文量

11364

期刊介绍： "Chinese Materia Medica" magazine was founded in 1978. It is a national-level academic journal of Chinese medicine science and technology approved by the State Science and Technology Commission and the Press and Publication Administration for public distribution at home and abroad. It is sponsored by the Chinese Materia Medica Information Center of the State Food and Drug Administration and is publicly distributed at home and abroad. It mainly reports on new technologies, new methods and new achievements in the production, scientific research and business management of Chinese medicine, and serves to promote the production and scientific and technological development of Chinese medicine, improve the output and quality of Chinese medicine, improve business management, and prosper the Chinese medicine industry.