Yu Liu, Jin Lan, Xin-ran Xu, Xiang-dong Chen, Chun-mei Yin
{"title":"[Comparison of Laccase Activity of Different Strains of Ganoderma spp.].","authors":"Yu Liu, Jin Lan, Xin-ran Xu, Xiang-dong Chen, Chun-mei Yin","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To explore the relationship between the laccase activity and growth of Ganoderma spp.,to screen the highproducing laccase strains.</p><p><strong>Methods: </strong>The mycelium was cultured in PDA solid medium,and the laccase activity was measured by ABTS+method. The content of polysaccharide was determined by phenol sulfuric acid method.</p><p><strong>Results: </strong>Ganoderma gibbosum grew more rapidly in the early stage,and the laccase activity reached maximum( 1 476. 64 U/g) in the 4th day,which was 11. 37 times larger than that of sweet Ganoderma lucidum. Ganoderma gibbosum had the significant advantage after a comprehensive analysis. The polysaccharide content of Ganoderma lucidum of Shinshu was lower; the growth rate of Ganoderma lucidum strain S3 was stable, and the content of polysaccharide was medium.</p><p><strong>Conclusion: </strong>There are some differences in the growth rate of Ganoderma spp., and there is a certain correlation between the laccase activity and growth. Ganoderma gibbosum is the highest-producing laccase strain among the screening strains.</p>","PeriodicalId":15312,"journal":{"name":"中药材","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"中药材","FirstCategoryId":"3","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Objective: To explore the relationship between the laccase activity and growth of Ganoderma spp.,to screen the highproducing laccase strains.
Methods: The mycelium was cultured in PDA solid medium,and the laccase activity was measured by ABTS+method. The content of polysaccharide was determined by phenol sulfuric acid method.
Results: Ganoderma gibbosum grew more rapidly in the early stage,and the laccase activity reached maximum( 1 476. 64 U/g) in the 4th day,which was 11. 37 times larger than that of sweet Ganoderma lucidum. Ganoderma gibbosum had the significant advantage after a comprehensive analysis. The polysaccharide content of Ganoderma lucidum of Shinshu was lower; the growth rate of Ganoderma lucidum strain S3 was stable, and the content of polysaccharide was medium.
Conclusion: There are some differences in the growth rate of Ganoderma spp., and there is a certain correlation between the laccase activity and growth. Ganoderma gibbosum is the highest-producing laccase strain among the screening strains.
期刊介绍:
"Chinese Materia Medica" magazine was founded in 1978. It is a national-level academic journal of Chinese medicine science and technology approved by the State Science and Technology Commission and the Press and Publication Administration for public distribution at home and abroad. It is sponsored by the Chinese Materia Medica Information Center of the State Food and Drug Administration and is publicly distributed at home and abroad. It mainly reports on new technologies, new methods and new achievements in the production, scientific research and business management of Chinese medicine, and serves to promote the production and scientific and technological development of Chinese medicine, improve the output and quality of Chinese medicine, improve business management, and prosper the Chinese medicine industry.