Detection of Plasmid-Mediated qnr Genes Among the Clinical Quinolone-Resistant Escherichia coli Strains Isolated in Tehran, Iran.

Q3 Immunology and Microbiology

Open Microbiology Journal Pub Date : 2018-07-31 eCollection Date: 2018-01-01 DOI:10.2174/1874285801812010248

Reza Ranjbar, Sajjad S Tolon, Mehrdad Sami, Reza Golmohammadi

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引用次数: 9

Abstract

Background: Escherichia coli is one of the most important bacterial agents to cause urinary tract infections. Inappropriate and unnecessary administration of antibiotics has led to an increase in the appearance of multidrug-resistant E. coli isolates, limiting treatment options. The increase in a number of resistant strains of bacteria is a major concern of health authorities worldwide.

Objective: The purpose of this study was to determine the presence of the qnr genes among E. coli isolated from UTIs of patients in Baqiyatallah hospital in Tehran province, Iran.

Method: Clinical urine samples of patients with suspected urinary tract infection were collected by standard methods in sterile disposable containers. After analysis of urine, microscopic observations and culture analysis, the bacterial genome was extracted by boiling method. PCR for detection of qnr genes including qnrA, qnrB and qnrS was done by specific primers, then PCR products were run using gel electrophoresis and visualized by gel documentation system.

Results: In the present study among the 95 isolates, 60 strains were resistant to nalidixic acid. PCR showed that 92 strains were positive for qnrS. The qnrA and qnrB genes were not found among the clinical isolates.

Conclusion: Our finding indicates a high level of resistance against nalidixic acid among E. coli isolates recovered from the patients with UTI. Also, the high frequency of qnrS imposes the importance of survey of molecular and genetic analysis of mechanisms of quinolone resistance in E. coli strains.

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伊朗德黑兰临床喹诺酮类耐药大肠埃希菌质粒介导qnr基因的检测

背景:大肠杆菌是引起尿路感染的重要细菌之一。不适当和不必要的抗生素使用导致耐多药大肠杆菌分离株的出现增加，限制了治疗选择。一些耐药细菌菌株的增加是世界各地卫生当局关注的一个主要问题。目的:研究伊朗德黑兰省Baqiyatallah医院尿路感染患者分离的大肠杆菌中qnr基因的存在。方法:对疑似尿路感染患者的临床尿样，按标准方法在一次性无菌容器中采集。经尿液分析、显微镜观察和培养分析后，采用煮沸法提取细菌基因组。PCR检测qnr基因，包括qnrA、qnrB和qnrS，然后用凝胶电泳和凝胶记录系统显示PCR产物。结果:95株菌株中有60株对萘啶酸耐药。PCR结果显示92株qnrS阳性。临床分离株中未发现qnrA和qnrB基因。结论:从尿路感染患者中分离出的大肠杆菌对萘啶酸具有较高的耐药性。此外，高频率的qnrS对大肠杆菌喹诺酮耐药机制的分子和遗传分析具有重要意义。

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来源期刊

Open Microbiology Journal Immunology and Microbiology-Immunology and Microbiology (all)

CiteScore

1.80

自引率

0.00%

发文量

期刊介绍： The Open Microbiology Journal is a peer-reviewed open access journal which publishes research articles, reviews/mini-reviews, case studies, guest edited thematic issues and short communications/letters covering theoretical and practical aspects of Microbial systematics, evolutionary microbiology, immunology, virology, parasitology , bacteriology, mycology, phycology, protozoology, microbial ecology, molecular biology, microbial physiology, biochemistry, microbial pathogenesis, host-microbe interaction, systems microbiology, synthetic microbiology, bioinformatics. The Open Microbiology Journal , a peer-reviewed journal, is an important and reliable source of current information on developments in the field. The emphasis will be on publishing quality papers rapidly and freely available to researchers worldwide.