Establishment of a detection assay for DNA endonuclease activity and its application in the screening and prognosis of malignant lymphoma.

Q2 Biochemistry, Genetics and Molecular Biology

BMC Biochemistry Pub Date : 2018-07-31 DOI:10.1186/s12858-018-0096-6

Renquan Lu, Yingchao Wang, Xiaofeng Xu, Suhong Xie, Yanchun Wang, Ailing Zhong, Hui Zheng, Yiwen Yu, Xiang Gao, Lin Guo

{"title":"Establishment of a detection assay for DNA endonuclease activity and its application in the screening and prognosis of malignant lymphoma.","authors":"Renquan Lu, Yingchao Wang, Xiaofeng Xu, Suhong Xie, Yanchun Wang, Ailing Zhong, Hui Zheng, Yiwen Yu, Xiang Gao, Lin Guo","doi":"10.1186/s12858-018-0096-6","DOIUrl":null,"url":null,"abstract":"Background: Endonucleases play critical roles in maintaining genomic stability and regulating cell growth. The purpose of this study was to evaluate detection of endonuclease activity as an indicator in the early diagnosis and prognosis of lymphoma.Results: The method of endonuclease activity determination was successfully established and applied to compare cancer patient and control cohorts. Endonuclease activities of cancer tissues were significantly higher than those of adjacent control tissues (P < 0.001). We next investigated endonuclease activity in peripheral blood of enrolled patients and the controls, which were also significantly higher in patients than in controls (P = 0.015). Additionally, endonuclease activities were elevated in the metastasis subgroup compared with the non-metastasis subgroup(P = 0.038), whereas no significant difference was found between age(≤ 56y, > 56y) and gender (P = 0.736 > 0.05 and P = 0.635 > 0.05, respectively). Although there was no significant difference between control group with the non-metastatic cancer patients (P = 0.800 > 0.05), endonuclease activities were lower in the control group compared with the non-metastatic cancer patients with lymphoma (P = 0.033). The progression-free survival probability of patients with elevated R ratios(R ratio ≥ 1.4) was significantly lower than that of patients with lower R ratios (R ratio < 1.4).Conclusions: An assay was established to detect the endonuclease activity,which might be useful for the prognosis of cancers, especially lymphoma.","PeriodicalId":9113,"journal":{"name":"BMC Biochemistry","volume":"19 1","pages":"6"},"PeriodicalIF":0.0000,"publicationDate":"2018-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6069817/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"BMC Biochemistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1186/s12858-018-0096-6","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Endonucleases play critical roles in maintaining genomic stability and regulating cell growth. The purpose of this study was to evaluate detection of endonuclease activity as an indicator in the early diagnosis and prognosis of lymphoma.

Results: The method of endonuclease activity determination was successfully established and applied to compare cancer patient and control cohorts. Endonuclease activities of cancer tissues were significantly higher than those of adjacent control tissues (P < 0.001). We next investigated endonuclease activity in peripheral blood of enrolled patients and the controls, which were also significantly higher in patients than in controls (P = 0.015). Additionally, endonuclease activities were elevated in the metastasis subgroup compared with the non-metastasis subgroup(P = 0.038), whereas no significant difference was found between age(≤ 56y, > 56y) and gender (P = 0.736 > 0.05 and P = 0.635 > 0.05, respectively). Although there was no significant difference between control group with the non-metastatic cancer patients (P = 0.800 > 0.05), endonuclease activities were lower in the control group compared with the non-metastatic cancer patients with lymphoma (P = 0.033). The progression-free survival probability of patients with elevated R ratios(R ratio ≥ 1.4) was significantly lower than that of patients with lower R ratios (R ratio < 1.4).

Conclusions: An assay was established to detect the endonuclease activity,which might be useful for the prognosis of cancers, especially lymphoma.

Abstract Image

查看原文本刊更多论文

建立 DNA 内切酶活性检测方法及其在恶性淋巴瘤筛查和预后中的应用。

背景：内切酶在维持基因组稳定性和调节细胞生长方面发挥着关键作用。本研究旨在评估内切酶活性检测作为淋巴瘤早期诊断和预后判断指标的作用：结果：成功建立了内切酶活性测定方法，并将其应用于癌症患者和对照组的比较。癌症组织的内切酶活性明显高于相邻对照组织（P 56y），性别也明显高于相邻对照组织（P = 0.736 > 0.05 和 P = 0.635 > 0.05）。虽然对照组与非转移性癌症患者无明显差异（P = 0.800 > 0.05），但对照组的内切酶活性低于非转移性癌症淋巴瘤患者（P = 0.033）。R比值升高（R比值≥1.4）的患者的无进展生存概率明显低于R比值较低的患者（R比值结论：R比值≥1.4）：建立了一种检测内切酶活性的方法，它可能对癌症尤其是淋巴瘤的预后有帮助。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

BMC Biochemistry BIOCHEMISTRY & MOLECULAR BIOLOGY-

CiteScore

4.80

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： BMC Biochemistry is an open access journal publishing original peer-reviewed research articles in all aspects of biochemical processes, including the structure, function and dynamics of metabolic pathways, supramolecular complexes, enzymes, proteins, nucleic acids and small molecular components of organelles, cells and tissues. BMC Biochemistry (ISSN 1471-2091) is indexed/tracked/covered by PubMed, MEDLINE, BIOSIS, CAS, EMBASE, Scopus, Zoological Record, Thomson Reuters (ISI) and Google Scholar.