{"title":"Microbiome Analyses for Toxicological Studies","authors":"Jacquelynn Benjamino, Lidia Beka, Joerg Graf","doi":"10.1002/cptx.53","DOIUrl":null,"url":null,"abstract":"Microbiomes can be thought of as the integration of biotic and abiotic factors, including the microbial communities and the distinct physio‐chemical properties that are present in a habitat. The microbes within a microbiome can influence the chemical environment by degrading medications inside patients or contaminants in the environment, such as hydrocarbons released after the Deepwater Horizon oil spill. Identifying and monitoring the relative abundance of microbes can help to elucidate variations in their response to toxins and should be considered as a variable in statistical analyses. In addition, the presence of pathogenic microbes or toxin‐producing species could affect the observed toxicity of an environment. The protocols in this unit begin with the collection of samples, proceeds to sequencing of the bacterial DNA, and ends with an analysis of the bacterial community in light of environmental metadata. © 2018 by John Wiley & Sons, Inc.","PeriodicalId":72743,"journal":{"name":"Current protocols in toxicology","volume":"77 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2018-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cptx.53","citationCount":"8","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current protocols in toxicology","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cptx.53","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 8
毒理学研究中的微生物组分析
微生物组可以被认为是生物和非生物因素的整合,包括存在于栖息地中的微生物群落和独特的物理化学特性。微生物组中的微生物可以通过降解患者体内的药物或环境中的污染物(如深水地平线石油泄漏后释放的碳氢化合物)来影响化学环境。识别和监测微生物的相对丰度有助于阐明它们对毒素反应的变化,并应被视为统计分析中的一个变量。此外,致病微生物或产生毒素的物种的存在也会影响环境的毒性。本单元的协议从收集样本开始,进行细菌DNA测序,并根据环境元数据分析细菌群落。©2018 by John Wiley &儿子,Inc。
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