Jessica R. Perez, Norma Ybarra, Frederic Chagnon, Olivier Lesur, Jan Seuntjens, Issam El Naqa
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Abstract
Stem cell therapy has shown great promise for organ repair and regeneration. In the context of lung disease, such as radiation-induced lung damage (RILD) in cancer radiotherapy, mesenchymal stem cells (MSCs) have shown the ability to reduce damage possibly due to their immunomodulatory properties and other unknown mechanisms. However, once MSCs are transplanted into the body, little is known as to their localization or their mechanisms of action. In this work, we proposed, implemented, and validated a fluorescence endomicroscopy (FE) imaging technique that allows for the real-time detection and quantification of transplanted pre-labeled MSCs in vivo and tracking in a rat model. This protocol covers aspects related to MSCs extraction, labeling, FE imaging, and image analysis developed in a RILD rat model but applicable to other biological systems. © 2018 by John Wiley & Sons, Inc.
大鼠肺间充质干细胞的荧光内镜成像
干细胞治疗在器官修复和再生方面显示出巨大的希望。在肺部疾病的背景下,如癌症放疗中的辐射诱导肺损伤(RILD),间充质干细胞(MSCs)已显示出减少损伤的能力,可能是由于其免疫调节特性和其他未知机制。然而,一旦骨髓间充质干细胞移植到体内,对其定位或作用机制知之甚少。在这项工作中,我们提出、实施并验证了一种荧光内镜(FE)成像技术,该技术可以实时检测和定量体内移植的预标记间充质干细胞,并在大鼠模型中进行跟踪。本协议涵盖了在RILD大鼠模型中开发的MSCs提取、标记、FE成像和图像分析相关的方面,但适用于其他生物系统。©2018 by John Wiley &儿子,Inc。
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