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{"title":"Clarity and Immunofluorescence on Mouse Brain Tissue","authors":"Tingting Yu, Dan Zhu","doi":"10.1002/cpns.46","DOIUrl":null,"url":null,"abstract":"<p>Tissue optical clearing techniques have provided essential tools for visualization of neural networks in unsectioned brain tissue. Here, we describe a passive optical clearing method based on hydrogel embedding, PACT (passive clarity technique), which is relatively simple. Advantages of PACT include tissue transparency, fluorescence preservation, and immunostaining compatibility for obtaining three-dimensional structures of mouse brain tissue. Additionally, it can enhance the penetration of antibodies in immunostaining and allows efficient immunolabeling of large volumes. After clearing with PACT, one can achieve high-resolution imaging in deep tissue at the millimeter-scale level with optical imaging microscopy. © 2018 by John Wiley & Sons, Inc.</p>","PeriodicalId":40016,"journal":{"name":"Current Protocols in Neuroscience","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2018-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpns.46","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Protocols in Neuroscience","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cpns.46","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Neuroscience","Score":null,"Total":0}
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Abstract
Tissue optical clearing techniques have provided essential tools for visualization of neural networks in unsectioned brain tissue. Here, we describe a passive optical clearing method based on hydrogel embedding, PACT (passive clarity technique), which is relatively simple. Advantages of PACT include tissue transparency, fluorescence preservation, and immunostaining compatibility for obtaining three-dimensional structures of mouse brain tissue. Additionally, it can enhance the penetration of antibodies in immunostaining and allows efficient immunolabeling of large volumes. After clearing with PACT, one can achieve high-resolution imaging in deep tissue at the millimeter-scale level with optical imaging microscopy. © 2018 by John Wiley & Sons, Inc.
小鼠脑组织的清晰度和免疫荧光
组织光学清除技术为未切片脑组织中神经网络的可视化提供了必要的工具。本文介绍了一种相对简单的基于水凝胶包埋的被动光学清除方法PACT (passive clarity technique)。PACT的优点包括组织透明、荧光保存和免疫染色兼容性，可以获得小鼠脑组织的三维结构。此外，它可以增强免疫染色中抗体的渗透，并允许大容量的有效免疫标记。在PACT清除后，人们可以在光学成像显微镜下实现毫米级的深部组织高分辨率成像。©2018 by John Wiley &儿子,Inc。
本文章由计算机程序翻译，如有差异，请以英文原文为准。
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