Monica Manglani, Selamawit Gossa, Dorian B. McGavern
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引用次数: 21
Abstract
A continual dialogue exists between the central nervous system (CNS) and immune system that contributes to neural homeostasis as well as protection from microbes, repair following damage, autoimmune disease, and neurodegeneration. Characterization of resident and peripherally derived leukocyte populations within the central nervous system can provide valuable information regarding how these cells contribute to steady-state and inflammatory conditions. Flow cytometry provides a method to conduct detailed multi-parameter analyses of immune cells isolated from various tissues. This protocol provides a method to isolate leukocytes from brain, spinal cord, and meninges for flow cytometric analysis and provides a basic framework for phenotyping these cells. © 2018 by John Wiley & Sons, Inc.
从脑、脊髓和脑膜分离白细胞用于流式细胞术分析
中枢神经系统(CNS)和免疫系统之间存在持续的对话，有助于神经稳态，保护微生物，修复损伤，自身免疫性疾病和神经退行性变。表征中枢神经系统内的常驻和外周来源的白细胞群可以提供有关这些细胞如何促进稳态和炎症条件的有价值的信息。流式细胞术提供了一种对从各种组织中分离的免疫细胞进行详细多参数分析的方法。该方案提供了一种从脑、脊髓和脑膜中分离白细胞的方法，用于流式细胞术分析，并为这些细胞的表型分析提供了基本框架。©2018 by John Wiley &儿子,Inc。
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