Mobile Quantitative Colocalization Analysis of Fluorescence Microscopy Images

Abstract

Understanding the nature of fluorescently-labelled molecules requires proper quantification of their colocalization. We developed a new approach to enable quantification of colocalization of markers in fluorescence microscopy images using mobile computers. It consists of three interacting components: desktop computer – cloud – mobile device. After an image is opened on a desktop computer, it is then saved to the cloud and becomes available to a mobile device. Functionality of the desktop and mobile software consists of the same steps and therefore allows using any of the platforms when performing analysis. Changes of the state of the image between devices are synchronized via the cloud, so that nothing is lost when switching them. All interactions are performed seamlessly in the background and do not require any input from the researchers’ side. This approach augments access to analytical imaging by allowing to work in completely new ways with superior levels of control, simplicity, and convenience. © 2018 by John Wiley & Sons, Inc.