Expression and Purification of Recombinant Proteins Using the Baculovirus System

Q2 Biochemistry, Genetics and Molecular Biology

Current Protocols in Molecular Biology Pub Date : 2018-06-28 DOI:10.1002/cpmb.61

Cheryl Isaac Murphy, Helen Piwnica-Worms, Stefan Grünwald, William G. Romanow, Nicole Francis, Hua-Ying Fan, Sharon Marr

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Abstract

This article describes how to analyze protein expression in cells infected with recombinant baculovirus on a small scale for optimizing protein production, how to maximize and scale up recombinant protein production, and how to purify recombinant proteins. © 2018 by John Wiley & Sons, Inc.

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杆状病毒系统中重组蛋白的表达与纯化

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current Protocols in Molecular Biology Biochemistry, Genetics and Molecular Biology-Molecular Biology

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