Top-down proteomic characterization of DAOY medulloblastoma tumor cell line

Q4 Biochemistry, Genetics and Molecular Biology

EuPA Open Proteomics Pub Date : 2016-09-01 DOI:10.1016/j.euprot.2016.03.015

Claudia Martelli , Luca D’Angelo , Marta Barba , Mirko Baranzini , Ilaria Inserra , Federica Iavarone , Federica Vincenzoni , Gianpiero Tamburrini , Luca Massimi , Concezio Di Rocco , Massimo Caldarelli , Irene Messana , Fabrizio Michetti , Massimo Castagnola , Wanda Lattanzi , Claudia Desiderio

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引用次数: 3

Abstract

The proteome of the DAOY medulloblastoma cell line has been investigated by an LC–MS top-down platform. This approach, unlike bottom-up ones, allows identifying proteins and peptides in their intact/native forms, disclosing post-translational modifications, proteoforms and naturally occurring peptides. Indeed, 25 out of the 53 proteins identified, were not previously characterized in DAOY cells. Most of them were functionally interconnected, being mainly involved in binding, catalytic and structural activities, and metabolic processes. The top-down approach, applied in this preliminary study, disclosed the presence of several naturally occurring peptide fragments that characterize DAOY cells.

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自顶向下的成神经管细胞瘤细胞系的蛋白质组学特征

采用LC-MS自顶向下平台研究了道伊成神经管细胞瘤细胞系的蛋白质组。与自下而上的方法不同，这种方法可以识别完整/天然形式的蛋白质和肽，揭示翻译后修饰，蛋白质形式和天然存在的肽。事实上，在鉴定的53种蛋白质中，有25种以前没有在dao细胞中被表征。它们大多在功能上相互联系，主要参与结合、催化和结构活动以及代谢过程。在这项初步研究中应用的自上而下的方法揭示了几种天然存在的多肽片段的存在，这些片段是day细胞的特征。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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EuPA Open Proteomics Biochemistry, Genetics and Molecular Biology-Biochemistry

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103 days