Marisa Maia , Filipa Monteiro , Mónica Sebastiana , Ana Patrícia Marques , António E.N. Ferreira , Ana Ponces Freire , Carlos Cordeiro , Andreia Figueiredo , Marta Sousa Silva
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引用次数: 34
Abstract
In metabolomics there is an ever-growing need for faster and more comprehensive analysis methods to cope with the increase of biological studies. Direct infusion Fourier-transform ion cyclotron-resonance mass spectrometry (DI-FTICR-MS) is used in non-targeted metabolomics to obtain high-resolution snapshots of the metabolic state of a system. In any metabolic profiling study, the establishment of an effective metabolite extraction protocol is paramount. We developed an improved metabolite extraction method, compatible with DI-FTICR-MS-based metabolomics, using grapevine leaves. This extraction protocol allowed the extraction of polar and non-polar compounds, covering all major classes found in plants and increasing metabolome coverage.
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