Development of a Bead-Based Multiplex Assay for the Analysis of the Serological Response against the Six Pathogens HAV, HBV, HCV, CMV, T. gondii, and H. pylori.

Q2 Biochemistry, Genetics and Molecular Biology

High-Throughput Pub Date : 2017-10-30 DOI:10.3390/ht6040014

Angela Filomena, Frank Pessler, Manas K Akmatov, Gérard Krause, Darragh Duffy, Barbara Gärtner, Markus Gerhard, Matthew L Albert, Thomas O Joos, Nicole Schneiderhan-Marra

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引用次数: 6

Abstract

The spread of infectious diseases and vaccination history are common subjects of epidemiological and immunological research studies. Multiplexed serological assays are useful tools for assessing both current and previous infections as well as vaccination efficacy. We developed a serological multi-pathogen assay for hepatitis A, B and C virus, cytomegalovirus (CMV), Toxoplasma gondii, and Helicobacter pylori using a bead-based multiplex assay format. The multi-pathogen assay consisting of 15 antigens was utilized for the analysis of the serological response in elderly individuals of an influenza vaccination study (n = 34). The technical assay validation revealed a mean intra-assay precision of coefficient of variation (CV) = 3.2 ± 1.5% and a mean inter-assay precision of CV = 8.2 ± 5.3% across all 15 antigens and all tested samples, indicating a robust test system. Furthermore, the assay shows high sensitivities (ranging between 94% and 100%) and specificities (ranging between 93% and 100%) for the different pathogens. The highest seroprevalence rates in our cohort were observed for hepatitis A virus (HAV; 73.5%), followed by CMV (70.6%), T. gondii (67.6%) and H. pylori (32.4%). Seroprevalences for hepatitis B virus (HBV, 8.8%) and hepatitis C virus (HCV, 0%) were low. The seroprevalences observed in our study were similar to those from other population-based studies in Germany. In summary, we conclude that our multiplex serological assay represents a suitable tool for epidemiological studies.

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建立一种基于小球的多重检测方法，用于分析对HAV、HBV、HCV、CMV、弓形虫和幽门螺杆菌六种病原体的血清学反应。

传染病的传播和疫苗接种史是流行病学和免疫学研究的共同主题。多重血清学检测是评估当前和既往感染以及疫苗接种效果的有用工具。我们开发了一种针对甲型肝炎、乙型肝炎和丙型肝炎病毒、巨细胞病毒(CMV)、刚地弓形虫和幽门螺杆菌的血清学多病原体检测方法，采用基于头部的多重检测格式。由15种抗原组成的多病原体试验被用于分析老年人流感疫苗接种研究的血清学反应(n = 34)。技术验证表明，15种抗原和所有检测样品的平均测定内变异系数(CV)的平均精密度为3.2±1.5%，平均测定间精密度为8.2±5.3%，表明检测系统的可靠性。此外，该分析对不同的病原体显示出高灵敏度(范围在94%至100%之间)和特异性(范围在93%至100%之间)。在我们的队列中，甲肝病毒(HAV)的血清阳性率最高;其次是巨细胞病毒(70.6%)、弓形虫(67.6%)和幽门螺旋杆菌(32.4%)。乙型肝炎病毒(HBV, 8.8%)和丙型肝炎病毒(HCV, 0%)的血清患病率较低。在我们的研究中观察到的血清患病率与德国其他基于人群的研究相似。总之，我们得出结论，我们的多重血清学检测是流行病学研究的合适工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

High-Throughput Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

3.60

自引率

0.00%

发文量

审稿时长

9 weeks

期刊介绍： High-Throughput (formerly Microarrays, ISSN 2076-3905) is a multidisciplinary peer-reviewed scientific journal that provides an advanced forum for the publication of studies reporting high-dimensional approaches and developments in Life Sciences, Chemistry and related fields. Our aim is to encourage scientists to publish their experimental and theoretical results based on high-throughput techniques as well as computational and statistical tools for data analysis and interpretation. The full experimental or methodological details must be provided so that the results can be reproduced. There is no restriction on the length of the papers. High-Throughput invites submissions covering several topics, including, but not limited to: -Microarrays -DNA Sequencing -RNA Sequencing -Protein Identification and Quantification -Cell-based Approaches -Omics Technologies -Imaging -Bioinformatics -Computational Biology/Chemistry -Statistics -Integrative Omics -Drug Discovery and Development -Microfluidics -Lab-on-a-chip -Data Mining -Databases -Multiplex Assays