Role of Farnesoid X Receptor in the Determination of Liver Transcriptome during Postnatal Maturation in Mice.

Nuclear Receptor Research Pub Date : 2017-01-01 Epub Date: 2017-10-20 DOI:10.11131/2017/101308
Lai Peng, Stephanie C Piekos, Grace L Guo, Xiao-Bo Zhong
{"title":"Role of Farnesoid X Receptor in the Determination of Liver Transcriptome during Postnatal Maturation in Mice.","authors":"Lai Peng, Stephanie C Piekos, Grace L Guo, Xiao-Bo Zhong","doi":"10.11131/2017/101308","DOIUrl":null,"url":null,"abstract":"<p><p>The liver is a vital organ with critical functions in metabolism of various biologically useful materials, synthesis of several vital proteins, detoxification of toxic substances, and immune defense. Most liver functions are not mature at birth and many changes happen during postnatal liver development, which lead to differential vulnerabilities of the liver at different developmental stages. However, the details of what changes occur in liver after birth, at what developmental stages they occur, and molecular mechanisms in the regulation of the developmental process are not clearly known. The nuclear receptor Farnesoid X receptor (FXR) is an important transcriptional regulator in liver. Here, we used RNA-Sequencing to analyze the transcriptome of mouse liver from perinatal to adult ages in both C57BL/6 and <i>Fxr</i><sup>-/-</sup> mice. We have defined a clear timeline of functional transition from prenatal through neonatal and adolescent to adult in C57BL/6 mice. Without FXR, activation of neonatal-specific pathways was prolonged and maturation of multiple metabolic pathways was delayed. The loss of FXR also led to increased expression of 27 other transcription regulators. Our data support a conclusion that developmental transcriptome revealed significant functional transition during postnatal liver development and FXR plays an important role in control of postnatal liver maturation.</p>","PeriodicalId":30720,"journal":{"name":"Nuclear Receptor Research","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5962295/pdf/","citationCount":"7","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nuclear Receptor Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.11131/2017/101308","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2017/10/20 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 7

Abstract

The liver is a vital organ with critical functions in metabolism of various biologically useful materials, synthesis of several vital proteins, detoxification of toxic substances, and immune defense. Most liver functions are not mature at birth and many changes happen during postnatal liver development, which lead to differential vulnerabilities of the liver at different developmental stages. However, the details of what changes occur in liver after birth, at what developmental stages they occur, and molecular mechanisms in the regulation of the developmental process are not clearly known. The nuclear receptor Farnesoid X receptor (FXR) is an important transcriptional regulator in liver. Here, we used RNA-Sequencing to analyze the transcriptome of mouse liver from perinatal to adult ages in both C57BL/6 and Fxr-/- mice. We have defined a clear timeline of functional transition from prenatal through neonatal and adolescent to adult in C57BL/6 mice. Without FXR, activation of neonatal-specific pathways was prolonged and maturation of multiple metabolic pathways was delayed. The loss of FXR also led to increased expression of 27 other transcription regulators. Our data support a conclusion that developmental transcriptome revealed significant functional transition during postnatal liver development and FXR plays an important role in control of postnatal liver maturation.

Abstract Image

Abstract Image

Abstract Image

法脂类X受体在小鼠出生后成熟过程中肝脏转录组测定中的作用。
肝脏是人体的重要器官,在各种生物有用物质的代谢、几种重要蛋白质的合成、有毒物质的解毒和免疫防御等方面具有重要功能。大多数肝脏功能在出生时并不成熟,在出生后肝脏发育过程中会发生许多变化,这导致肝脏在不同发育阶段的脆弱性存在差异。然而,出生后肝脏发生了哪些变化,发生在哪个发育阶段,以及发育过程中调控的分子机制等细节尚不清楚。核受体Farnesoid X受体(FXR)是肝脏重要的转录调节因子。在这里,我们使用rna测序分析了C57BL/6和Fxr-/-小鼠从围产期到成年的小鼠肝脏转录组。我们已经明确了C57BL/6小鼠从产前到新生儿、青春期到成年的功能转变时间表。没有FXR,新生儿特异性通路的激活被延长,多种代谢通路的成熟被延迟。FXR的缺失也导致27个其他转录调控因子的表达增加。我们的数据支持一个结论,发育转录组揭示了出生后肝脏发育过程中显著的功能转变,FXR在控制出生后肝脏成熟中起重要作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
审稿时长
12 weeks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信