[Gene cloning and biological function of CgRGS2 in Colletotrichum gloeosporioides].

微生物学报 Pub Date : 2017-01-04

Manli Wu, Xiaoyu Li, Nan Zhang, Shuang Xu, Zhiqiang Liu

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引用次数: 0

Abstract

Objective: Regulators of G-protein signaling (RGS) are negative regulatory factors of G protein and play important roles in growth development and pathogenicity of plant pathogen. However, biological functions of RGS in Colletotrichum gloeosporioides have not been studied so far. We cloned an RGS gene of CgRGS2 in C. gloeosporioides and analyzed its biological function.

Methods: Gene CgRGS2 was cloned using PCR and analyzed. The gene-knockout mutant of CgRGS2 was obtained by homologous recombination, and the complementary strain was also built based on the mutant. Biological function of CgRGS2 was determined through phenotypic analysis.

Results: CgRGS2 encoded a 574-amino acids protein, containing an RGS function domain in the N terminal. Comparing to the wild type, the knockout mutant of CgRGS2 had slow growth, thick aerial hyphae, reduced conidia with multi-end germination, sensitive to oxidative stress and SDS, decreased pathogenicity.

Conclusion: Protein CgRGS2 was involved in regulation of vegetative growth, conidium production and germination, oxidative stress response, cell wall integrity and pathogenicity of C. gloeosporioides.

本刊更多论文

[炭疽菌CgRGS2基因克隆及生物学功能研究]。

目的:G蛋白信号调控因子(regulatory of G-protein signaling, RGS)是G蛋白的负调控因子，在植物病原菌的生长发育和致病性中起重要作用。然而，RGS在炭疽菌中的生物学功能尚未得到研究。我们克隆了一种CgRGS2的RGS基因，并分析了其生物学功能。方法:采用PCR方法克隆CgRGS2基因并进行分析。通过同源重组获得CgRGS2基因敲除突变体，并以该突变体为基础构建互补菌株。通过表型分析确定CgRGS2的生物学功能。结果:CgRGS2编码了一个574个氨基酸的蛋白，在N端含有一个RGS功能域。与野生型相比，CgRGS2基因敲除突变体生长缓慢，气生菌丝粗大，分生孢子多端萌发减少，对氧化应激和SDS敏感，致病性降低。结论:CgRGS2蛋白参与了gloeosporioides营养生长、分生孢子产生和萌发、氧化应激反应、细胞壁完整性和致病性的调控。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

微生物学报

自引率

0.00%

发文量

7960

期刊介绍： Acta Microbiologica Sinica(AMS) is a peer-reviewed monthly (one volume per year)international journal,founded in 1953.It covers a wide range of topics in the areas of general and applied microbiology.The journal publishes original papers,reviews in microbiological science,and short communications describing unusual observations. Acta Microbiologica Sinica has been indexed in Index Copernicus (IC),Chemical Abstract (CA),Excerpt Medica Database (EMBASE),AJ of Viniti (Russia),Biological Abstracts (BA),Chinese Science Citation Database (CSCD),China National Knowledge Infrastructure(CNKI),Institute of Scientific and Technical Information of China(ISTIC),Chinese Journal Citation Report(CJCR),Chinese Biological Abstracts,Chinese Pharmaceutical Abstracts,Chinese Medical Abstracts and Chinese Science Abstracts.