[Diversity and antimicrobial activities of cultivable bacteria isolated from Jiaozhou Bay].

微生物学报 Pub Date : 2016-12-04

Yiting Wang, Chuanbo Zhang, Lin Qi, Xiaoqiang Jia, Wenyu Lu

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引用次数: 0

Abstract

Objective: Marine microorganisms have a great potential in producing biologically active secondary metabolites. In order to study the diversity and antimicrobial activity, we explored 9 sediment samples in different observation sites of Jiaozhou bay.

Methods: We used YPD and Z2216E culture medium to isolate bacteria from the sediments; 16S rRNA was sequenced for classification and identification of the isolates. Then, we used Oxford cup method to detect antimicrobial activities of the isolated bacteria against 7 test strains. Lastly, we selected 16 representatives to detect secondary-metabolite biosynthesis genes:PKSI, NRPS, CYP, PhzE, dTGD by PCR specific amplification.

Results: A total of 76 bacterial strains were isolated from Jiaozhou bay; according to the 16S rRNA gene sequence analysis. These strains could be sorted into 11 genera belonging to 8 different families:Aneurinibacillus, Brevibacillus, Microbacterium, Oceanisphae, Bacillus, Marinomonas, Staphylococcus, Kocuria, Arthrobacters, Micrococcus and Pseudoalteromonas. Of them 34 strains showed antimicrobial activity against at least one of the tested strains. All 16 strains had at least one function genes, 5 strains possessed more than three function genes.

Conclusion: Jiaozhou bay area is rich in microbial resources with potential in providing useful secondary metabolites.

本刊更多论文

[胶州湾可培养细菌多样性及抑菌活性研究]。

目的:海洋微生物在产生具有生物活性的次生代谢产物方面具有很大的潜力。为了研究胶州湾不同观测点的9个沉积物样品的多样性和抗菌活性。方法:采用YPD和Z2216E培养基从沉积物中分离细菌;采用16S rRNA测序对分离株进行分类鉴定。然后采用牛津杯法检测分离菌对7种试验菌株的抑菌活性。最后，我们选择了16个代表性的二级代谢物生物合成基因:PKSI、NRPS、CYP、PhzE、dTGD，通过PCR特异性扩增进行检测。结果:从胶州湾共分离到76株细菌;根据16S rRNA基因序列分析。这些菌株可分为8科11属:动脉瘤芽孢杆菌、短芽孢杆菌、微杆菌、海洋芽孢杆菌、芽孢杆菌、海洋单胞菌、葡萄球菌、Kocuria、关节菌、微球菌和假互单胞菌。其中34株对至少一种受试菌株表现出抑菌活性。16株菌株均具有至少1个功能基因，5株菌株具有3个以上功能基因。结论:胶州湾地区微生物资源丰富，具有提供有益次生代谢产物的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

微生物学报

自引率

0.00%

发文量

7960

期刊介绍： Acta Microbiologica Sinica(AMS) is a peer-reviewed monthly (one volume per year)international journal,founded in 1953.It covers a wide range of topics in the areas of general and applied microbiology.The journal publishes original papers,reviews in microbiological science,and short communications describing unusual observations. Acta Microbiologica Sinica has been indexed in Index Copernicus (IC),Chemical Abstract (CA),Excerpt Medica Database (EMBASE),AJ of Viniti (Russia),Biological Abstracts (BA),Chinese Science Citation Database (CSCD),China National Knowledge Infrastructure(CNKI),Institute of Scientific and Technical Information of China(ISTIC),Chinese Journal Citation Report(CJCR),Chinese Biological Abstracts,Chinese Pharmaceutical Abstracts,Chinese Medical Abstracts and Chinese Science Abstracts.