[Isolation and characterization of a manganese-oxidizing bacterium from soils].

微生物学报 Pub Date : 2016-11-04
Yi Wang, Peng Xu, Xin Dai
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引用次数: 0

Abstract

Objective: The aim of this study was to isolate and characterize manganese (Ⅱ)-oxidizing bacteria from surrounding area of manganese mine.

Methods: Mn (Ⅱ)-oxidizing strains were isolated based on the ability to produce brown Mn oxides on agar plates. The presence of Mn oxides was confirmed by using the leucoberbelin blue (LBB) assay. The isolate was identified by morphological and physiological characterization and sequence analyses of 16S rRNA gene, gyrB gene and gyrA gene. The phylogenetic relationship between the isolated strain and reported Mn (Ⅱ) oxidizers was also analyzed. LBB assay was used to indicate the kinetics of Mn (Ⅱ) oxides formation. The Mn oxides morphology and chemical contents were determined by scanning electron microscope with energy dispersive spectrometer and X-ray diffraction.

Results: An isolate, named strain CP133, with high manganese oxidizing activity was obtained and identified as Bacillus cereus. There were some phylogenetic differences between strain CP133 and other Mn (Ⅱ)-oxidizing Bacillus species isolated from deep sea and soils. Strain CP133 produced amorphous manganese oxides that adhered to spore surface after the stationary phase of the cell culture. About 0.3 mmol/L manganese oxides was obtained after 12 days.

Conclusion: A Mn (Ⅱ)-oxidizing Bacillus cereus was successfully isolated. Our results suggest the diversity of Mn (Ⅱ)-oxidizing Bacillus species, and help understanding biogeochemical cycles in manganese mine and surrounding soils.

[从土壤中分离和鉴定一种锰氧化细菌]。
目的:对锰矿周边地区的锰(Ⅱ)氧化菌进行分离和鉴定。方法:根据在琼脂平板上产生棕色锰氧化物的能力,分离Mn(Ⅱ)氧化菌株。用白蛋白蓝(LBB)测定法证实了锰氧化物的存在。通过形态学和生理特征分析以及16S rRNA基因、gyrB基因和gyrA基因的序列分析对分离物进行了鉴定。分析了分离菌株与已报道的Mn(Ⅱ)氧化剂的系统发育关系。用LBB法测定Mn(Ⅱ)氧化物形成动力学。用扫描电镜、能谱仪和x射线衍射仪测定了锰氧化物的形貌和化学成分。结果:获得一株具有高锰氧化活性的分离菌株CP133,鉴定为蜡样芽孢杆菌。CP133菌株与其他从深海和土壤中分离的Mn(Ⅱ)氧化芽孢杆菌存在一定的系统发育差异。菌株CP133在细胞培养的固定阶段产生了粘附在孢子表面的无定形氧化锰。12 d后,锰的氧化率约为0.3 mmol/L。结论:成功分离到了一株Mn(Ⅱ)氧化蜡样芽孢杆菌。研究结果揭示了锰(Ⅱ)氧化芽孢杆菌种类的多样性,有助于了解锰矿及其周围土壤的生物地球化学循环。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
自引率
0.00%
发文量
7960
期刊介绍: Acta Microbiologica Sinica(AMS) is a peer-reviewed monthly (one volume per year)international journal,founded in 1953.It covers a wide range of topics in the areas of general and applied microbiology.The journal publishes original papers,reviews in microbiological science,and short communications describing unusual observations. Acta Microbiologica Sinica has been indexed in Index Copernicus (IC),Chemical Abstract (CA),Excerpt Medica Database (EMBASE),AJ of Viniti (Russia),Biological Abstracts (BA),Chinese Science Citation Database (CSCD),China National Knowledge Infrastructure(CNKI),Institute of Scientific and Technical Information of China(ISTIC),Chinese Journal Citation Report(CJCR),Chinese Biological Abstracts,Chinese Pharmaceutical Abstracts,Chinese Medical Abstracts and Chinese Science Abstracts.
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