Antibacterial effect of propolis derived from tribal region on Streptococcus mutans and Lactobacillus acidophilus: An in vitro study.

Q3 Dentistry

Journal of the Indian Society of Pedodontics and Preventive Dentistry Pub Date : 2018-01-01 DOI:10.4103/JISPPD.JISPPD_1128_17

Bhuvnesh Airen, Priyanka Airen Sarkar, Urvashi Tomar, Kundendu Arya Bishen

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引用次数: 26

Abstract

Aim: The study aimed at investigating in vitro antimicrobial activity of ethanolic extract of propolis (EEP) and water extract of propolis against two main cariogenic oral pathogens: Streptococcus mutans and Lactobacillus acidophilus.

Material and methodology: Propolis was obtained from beehives in the Jhabua region of India. Ethanolic and water extracts were prepared at concentrations of 5% and 20% weight/volume (w/v). To support the results, a positive control (chlorhexidine 0.2%) and a negative control (distilled water) were used. S. mutans was cultured on brain-heart infusion agar and L. acidophilus was cultured on De Man, Rogosa, and Sharpe agar.

Results: The results showed that at concentrations of 5% and 20%, EEP was effective against S. mutans and L. acidophilus. However, at similar concentrations, water extract was effective only against L. acidophilus. The highest activity was shown by chlorhexidine (0.2%) with mean zones of inhibition of 13.9 mm and 15.1 mm against S. mutans and L. acidophilus, respectively.

Conclusion: It can be concluded that the propolis extracted from tribal regions of Jhabua possesses antibacterial efficacy against S. mutans and L. acidophilus.

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部落地区蜂胶对变形链球菌和嗜酸乳杆菌抑菌作用的体外研究。

目的:研究蜂胶醇提物(EEP)和蜂胶水提物对两种口腔致病菌变形链球菌和嗜酸乳杆菌的体外抑菌活性。材料和方法:蜂胶是从印度jhahua地区的蜂箱中获得的。分别以5%和20%重量/体积(w/v)的浓度制备乙醇和水提取物。为支持结果，采用阳性对照(0.2%氯己定)和阴性对照(蒸馏水)。在脑心灌注琼脂上培养变形链球菌，在De Man、Rogosa和Sharpe琼脂上培养嗜酸乳杆菌。结果:EEP在5%和20%浓度下对变形链球菌和嗜酸乳杆菌均有较好的抑菌效果。然而，在相同浓度下，水提取物仅对嗜酸乳杆菌有效。氯己定(0.2%)对变形链球菌和嗜酸乳杆菌的平均抑制区分别为13.9 mm和15.1 mm，活性最高。结论:嘉华部落地区提取的蜂胶对变形链球菌和嗜酸乳杆菌具有抗菌作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of the Indian Society of Pedodontics and Preventive Dentistry Dentistry-Dentistry (all)

CiteScore

1.50

自引率

0.00%

发文量

审稿时长

39 weeks

期刊介绍： Journal of Indian Society of Pedodontics and Preventive Dentistry (ISSN - 0970-4388) is the official organ of Indian Society of Pedodontics and Preventive Dentistry. The journal publishes original articles and case reports pertaining to pediatric and preventive dentistry.