Impaired Inflammatory Response to LPS in Type 2 Diabetes Mellitus.

IF 2.6 Q3 IMMUNOLOGY

International Journal of Inflammation Pub Date : 2018-01-14 eCollection Date: 2018-01-01 DOI:10.1155/2018/2157434

Lusine Khondkaryan, Sona Margaryan, David Poghosyan, Gayane Manukyan

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引用次数: 23

Abstract

Type 2 diabetes mellitus (T2DM) is a severe health problem worldwide, reaching epidemic levels. High susceptibility to infections of T2DM patients indicates dysregulated immune responses to pathogens. However, innate immune responses, including monocyte functions, in T2DM are poorly investigated. Therefore, in this study we aimed to assess lipopolysaccharide- (LPS-) induced immune responses of circulating monocytes from T2DM patients. The results showed that monocytes from T2DM were hyporesponsive to LPS challenge as reflected by significantly suppressed secretion of TNFα (p < 0.01) and expression of CD11b (p < 0.001) and TLR4 (p < 0.001) compared to those in monocytes from healthy subjects. Furthermore, LPS-induced IL-10 levels were similar in diabetic and healthy supernatants, while expression levels of CD163 were found to be downregulated on monocytes from T2DM (p < 0.001) suggesting impaired ability of monocytes to switch their phenotype to anti-inflammatory. Taken together, our results suggest compromised function of monocytes in T2DM, which may explain, at least partly, high incidence of infection in these patients.

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2型糖尿病患者对LPS的炎症反应受损。

2型糖尿病(T2DM)是世界范围内严重的健康问题，已达到流行病水平。T2DM患者对感染的高易感性表明对病原体的免疫反应失调。然而，先天免疫反应，包括单核细胞功能，在2型糖尿病的研究很少。因此，在本研究中，我们旨在评估脂多糖(LPS)诱导的T2DM患者循环单核细胞的免疫反应。结果显示，与健康人相比，T2DM单核细胞对LPS的反应较低，TNFα分泌(p < 0.01)、CD11b表达(p < 0.001)和TLR4表达(p < 0.001)明显受到抑制。此外，lps诱导的IL-10水平在糖尿病和健康上清中相似，而CD163在T2DM单核细胞中的表达水平下调(p < 0.001)，表明单核细胞将其表型转换为抗炎的能力受损。综上所述，我们的研究结果表明，T2DM患者单核细胞功能受损，这可能解释了，至少部分解释了这些患者感染发生率高的原因。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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