Development of an In Vitro Method to Estimate the Sensitization Induction Level of Contact Allergens

Valentina Galbiati, Sue Gibbs, Erwin Roggen, Emanuela Corsini
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引用次数: 16

Abstract

Recently, an in vitro procedure, which combines the epidermal equivalent potency assay with assessment of IL-18 to provide a single test for identification and classification of skin sensitizers, was developed and validated. This unit will describe a simple in vitro method for estimation of the expected sensitization induction level interpolating in vitro EC50 and IL-18 SI2 values to predict LLNA EC3 and/or human NOEL from standards curves generated using reference contact allergens, based on the use of Reconstituted human Epidermis (RhE).© 2018 by John Wiley & Sons, Inc.

一种评估接触过敏原致敏诱导水平的体外方法的建立
最近,研究人员开发并验证了一种体外程序,该程序将表皮等效效力测定与IL-18评估相结合,为皮肤致敏剂的鉴定和分类提供了一种单一的测试。本单元将描述一种简单的体外方法,用于估计预期的致敏诱导水平,通过体外EC50和IL-18 SI2值来预测LLNA EC3和/或人类NOEL,该方法基于使用重组人表皮(RhE)生成的参考接触过敏原标准曲线。©2018 by John Wiley &儿子,Inc。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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