Minocycline in Alcohol Withdrawal Induced Anxiety and Alcohol Relapse in Rats.

IF 3.2 Q2 Pharmacology, Toxicology and Pharmaceutics

Current clinical pharmacology Pub Date : 2018-01-01 DOI:10.2174/1574884713666180228110310

Snehalata V Gajbhiye, Raakhi K Tripathi, Anup Petare, Anirudha V Potey, Arun Shankar

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引用次数: 17

Abstract

Introduction: Anxiety and negative sensations due to alcohol withdrawal are factors leading to alcohol relapse and addiction. Minocycline, an antibiotic, can decrease alcohol consumption in rats, however, its effects on alcohol withdrawal anxiety and relapse have not been studied.

Material and methods: Part 1: Forced alcohol drinking in gradually increasing concentration was administered till day 22 in rats. Effect of drugs on anxiety was assessed using elevated plus maze (EPM) and two-chambered box apparatus, after removal of alcohol. Part 2: For relapse, an alcohol deprivation effect model was used, rats were continuously offered alcohol and water for 4 consecutive weeks in a two-bottle choice paradigm, followed by 2 weeks of alcohol deprivation. Effect of drugs on alcohol consumption during the first hour of alcohol reintroduction was assessed. Animals were sacrificed and whole brain Tumor Necrosis Factor (TNF) α was estimated.

Results: Part 1: Anxiety at 3 hours was significantly lower following minocycline (20 mg/kg i.p.) or diazepam compared to vehicle control. Part 2: Acute administration of minocycline (5,10 and 20 mg/kg, i.p.) suppressed alcohol consumption significantly (p value<0.05) as compared to vehicle control. A significant decrease in whole brain TNF α was observed in animals treated with minocycline compared to untreated animals.

Conclusion: Minocycline attenuates alcohol withdrawal anxiety and disrupts alcohol relapse.

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米诺环素对大鼠酒精戒断诱导的焦虑和酒精复发的影响。

酒精戒断引起的焦虑和负面感觉是导致酒精复发和成瘾的因素。米诺环素是一种抗生素，可以减少大鼠的酒精摄入量，然而，它对酒精戒断焦虑和复发的影响尚未被研究过。材料与方法:第1部分:大鼠按逐渐增加的浓度强制饮酒至第22天。在去除酒精后，采用升高+迷宫(EPM)和双室箱仪评估药物对焦虑的影响。第二部分:对于复发，采用酒精剥夺效应模型，在两瓶选择范式下连续4周连续给予大鼠酒精和水，然后连续2周酒精剥夺。评估药物对酒精重新引入的第一个小时内酒精消耗的影响。处死动物，测定全脑肿瘤坏死因子(TNF) α。结果:第一部分:与对照组相比，米诺环素(20mg /kg i.p)或地西泮治疗后3小时的焦虑显著降低。第二部分:急性给予米诺环素(5、10和20 mg/kg, i.p.)可显著抑制酒精消耗(p值)。结论:米诺环素可减轻酒精戒断焦虑并阻断酒精复发。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current clinical pharmacology PHARMACOLOGY & PHARMACY-

CiteScore

3.60

自引率

0.00%

发文量

期刊介绍： Current Clinical Pharmacology publishes frontier reviews on all the latest advances in clinical pharmacology. The journal"s aim is to publish the highest quality review articles in the field. Topics covered include: pharmacokinetics; therapeutic trials; adverse drug reactions; drug interactions; drug metabolism; pharmacoepidemiology; and drug development. The journal is essential reading for all researchers in clinical pharmacology.