Antimicrobial Activity of Bacillus Persicus 24-DSM Isolated from Dead Sea Mud.

Q3 Immunology and Microbiology

Open Microbiology Journal Pub Date : 2017-12-29 eCollection Date: 2017-01-01 DOI:10.2174/1874285801711010372

Nehaya Al-Karablieh

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引用次数: 6

Abstract

Intorduction: Dead Sea is a hypersaline lake with 34% salinity, gains its name due to the absence of any living macroscopic creatures. Despite the extreme hypersaline environment, it is a unique ecosystem for various halophilic microorganisms adapted to this environment.

Aims & objectives: Halophilic microorganisms are known for various potential biotechnological applications, the purpose of the current research is isolation and screening of halophilic bacteria from Dead Sea mud for potential antimicrobial applications.

Methods & materials: Screening for antagonistic bacteria was conducted by bacterial isolation from Dead Sea mud samples and agar plate antagonistic assay. The potential antagonistic isolates were subjected to biochemical characterization and identification by 16S-rRNA sequencing. Among the collected isolates, four isolates showed potential antagonistic activity against Bacillus subtilis 6633 and Escherichia coli 8739. The most active isolate (24-DSM) was subjected for antagonistic activity and minimal inhibitory concentration against different gram positive and negative bacterial strains after cultivation in different salt concentration media. Results: The results of 16S-rRNA analysis revealed that 24-DSM is very closely related to Bacillus persicus strain B48, which was isolated from hypersaline lake in Iran.

Conclusion: Therefore, the isolate 24-DSM is assigned as a new strain of B. persicusi isolated from the Dead Sea mud. B. persicusi 24-DSM showed higher antimicrobial activity, when it was cultivated with saline medium, against all tested bacterial strains, where the most sensitive bacterial strain was Corynebacterium diphtheria 51696.

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死海泥中鼠疫芽孢杆菌24-DSM的抑菌活性

简介:死海是一个盐度高达34%的高咸水湖，因没有任何宏观生物而得名。尽管极端的高盐环境，它是各种嗜盐微生物适应这种环境的独特生态系统。目的和目的:嗜盐微生物以各种潜在的生物技术应用而闻名，目前研究的目的是从死海泥中分离和筛选嗜盐细菌，以用于潜在的抗菌应用。方法与材料:采用死海泥样品细菌分离和琼脂平板拮抗实验进行拮抗细菌筛选。对潜在拮抗菌株进行了生化鉴定和16S-rRNA测序。其中4株菌株对枯草芽孢杆菌6633和大肠杆菌8739具有潜在的拮抗活性。在不同盐浓度培养基中培养后，活性最高的菌株(24-DSM)对不同革兰氏阳性和阴性菌株具有拮抗活性，抑菌浓度最低。结果:16S-rRNA分析结果显示，24-DSM与来自伊朗高盐湖的芽孢杆菌B48亲缘关系密切。结论:该分离物24-DSM可确定为死海泥中分离到的一株新菌株。白喉棒状芽孢杆菌24-DSM在生理盐水培养基中培养时对所有菌株均有较高的抑菌活性，其中对白喉棒状芽孢杆菌51696最敏感。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Open Microbiology Journal Immunology and Microbiology-Immunology and Microbiology (all)

CiteScore

1.80

自引率

0.00%

发文量

期刊介绍： The Open Microbiology Journal is a peer-reviewed open access journal which publishes research articles, reviews/mini-reviews, case studies, guest edited thematic issues and short communications/letters covering theoretical and practical aspects of Microbial systematics, evolutionary microbiology, immunology, virology, parasitology , bacteriology, mycology, phycology, protozoology, microbial ecology, molecular biology, microbial physiology, biochemistry, microbial pathogenesis, host-microbe interaction, systems microbiology, synthetic microbiology, bioinformatics. The Open Microbiology Journal , a peer-reviewed journal, is an important and reliable source of current information on developments in the field. The emphasis will be on publishing quality papers rapidly and freely available to researchers worldwide.