Age-Dependent Susceptibility to Alcohol-Induced Cerebral Artery Constriction.

Q4 Psychology

Journal of Drug and Alcohol Research Pub Date : 2016-01-01 Epub Date: 2016-11-27 DOI:10.4303/jdar/236002

Anna N Bukiya, Olga Seleverstov, Shivantika Bisen, Alex M Dopico

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Abstract

Background: Age has been recognized as an important contributor into susceptibility to alcohol-driven pathology.

Purpose: We aimed at determining whether alcohol-induced constriction of cerebral arteries was age-dependent.

Study design: We used rat middle cerebral artery (MCA) in vitro diameter monitoring, patch-clamping and fluorescence labeling of myocytes to study an age-dependent increase in the susceptibility to alcohol in 3 (50 g), 8 (250 g), and 15 (440 g) weeks-old rats.

Results: An age-dependent increase in alcohol-induced constriction of MCA could be observed in absence of endothelium, which is paralleled by an age-dependent increase in both protein level of the calcium-/voltage-gated potassium channel of large conductance (BK) accessory β1 subunit and basal BK channel activity. Ethanol-induced BK channel inhibition is increased with age.

Conclusions: We demonstrate an increased susceptibility of MCA to ethanol-induced constriction in a period equivalent to adolescence and early adulthood when compared to pre-adolescence. Our work suggests that BK β1 constitutes a significant contributor to age-dependent changes in the susceptibility of cerebral arteries to ethanol.

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酒精诱发脑动脉收缩的易感性与年龄有关

背景：目的：我们旨在确定酒精诱导的脑动脉收缩是否与年龄有关：研究设计：我们使用大鼠大脑中动脉（MCA）体外直径监测、膜片钳和肌细胞荧光标记技术，研究了3周龄（50克）、8周龄（250克）和15周龄（440克）大鼠对酒精的敏感性随年龄增长的变化：结果：在没有内皮的情况下，可以观察到酒精诱导的 MCA 收缩随年龄的增加而增加，与此同时，大电导（BK）附属β1 亚基的钙/电压门控钾通道蛋白水平和基础 BK 通道活性也随年龄的增加而增加。乙醇诱导的 BK 通道抑制随年龄增长而增加：我们的研究表明，与青春期前相比，在青春期和成年早期，MCA 对乙醇诱导的收缩的敏感性增加。我们的研究表明，BK β1是脑动脉对乙醇的易感性随年龄变化而变化的一个重要因素。

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来源期刊

Journal of Drug and Alcohol Research Psychology-Clinical Psychology

CiteScore

0.50

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0.00%

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期刊介绍： The Journal of Drug and Alcohol Research (JDAR) is a scholarly open access, peer-reviewed, and fully refereed journal dedicated to publishing sound papers on advances in the field of drug, opiate, nicotine and alcohol abuse, both basic and clinical. The journal will consider papers from all sub-disciplines and aspects of drug abuse, dependence and addiction research. Manuscripts will be published online as soon as they are accepted, which will reduce the time of publication. Because there are no space limitations or favored topics, all papers, within the scope of the journal, judged to be sound by the reviewers, will be published.