Monitoring of brightness temperature of suspension of follicular thyroid carcinoma cells in SHF range by radiothermometry.

Abstract

The purpose: The purpose of this research consisted in monitoring of brightness temperature of the suspension of follicular thyroid carcinoma cells during the necrosis of these cells in superhigh frequency (SHF) range.

Methods: The monitoring of the change in the ratio between brightness temperatures TSHF and TIR values during the necrosis of these cells. The object of study was follicular thyroid carcinoma cells suspension prepared with use of Versene solution and 0.25% trypsin solution. The cells were precipitated by centrifugation and re-suspended in culture medium. The measurements of brightness temperatures were carried out with use of radiothermoimeter. SHF range was 3.4-4.2 GHz, and infrared (IR) range was 8-13 mm. The temperature of the suspension was maintained at 37.5°С.

Results: It was found that upon the necrosis in the suspension of cells, an increase in brightness temperature in 3.4-4.2 GHz range (SHF range) occurs, while brightness temperature of the medium in the IR range does not change.

Conclusion: The monitoring of necrosis of follicular thyroid carcinoma cells was carried out by SHF-radiothermometry. It was shown that during the necrosis the change of non-equilibrium state of cell medium occurs, that results in the change in the ratio between TSHF and TIR. During the necrosis, the brightness temperature in SHF range (TSHF) increases.