Features purine metabolism in patients with pulmonary tuberculosis.

M E Dyakova
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Abstract

The purpose - comprehensive study of the purine metabolic enzymes in serum and immune cells in patients with pulmonary tuberculosis for the understanding of the pathogenesis of a specific lung disease.

Methods: The enzymes of purine metabolism (adenosine deaminase (ADA) and its isoenzymes (ADA-1 and ADA-2), dipeptidylpeptidase IV (DPPIV - CD26), ecto-5’-nucleotidase (5’-NC) in the blood and immune cells was studied in 29 and 76 patients with fibro-cavernous (FCPT) and infiltrative (IPT) pulmonary tuberculosis correspondingly.

Results: In patients found changes in purine metabolism, the severity and pathophysiological significance of which depend of clinical forms of tuberculosis, that is, from the gravity specific of the process. Reduced activity of ADA mononuclear cells was accompanied by a decrease in the expression of CD26 in patients with FCPT and the growth of the IPT ectopeptidase patients, that is, the concentration of CD26 mononuclear cells and neutrophils are associated with form of pulmonary tuberculosis. The increased levels of another enzyme purine metabolism - 5’-NC registered in both forms of pulmonary tuberculosis.

Conclusion: In the context of the ADA and CD26 association with the IPT can assume increased participation of each of them in the activation of cell proliferation and cytokine production. Low levels of CD26 immune cells in the absence of their connection with the activity of ADA is typical for patients with FCPT and reflects their inherent failure of cellular immunity. We can assume that the formation of complexes with the ADA ectopeptidases (CD26 and 5’-NC) for newly diagnosed IPT provides a balance CD26_ADA extracellular / intracellular adenosine and 5’-NC / adenosine and thereby adequate metabolism of immunocompetent cells.

肺结核患者嘌呤代谢的特征。
目的:全面研究肺结核患者血清和免疫细胞中的嘌呤代谢酶,以了解特定肺部疾病的发病机制。方法:分别对29例和76例纤维海绵状肺结核(FCPT)和浸润性肺结核(IPT)患者血液和免疫细胞中的嘌呤代谢酶(腺苷脱氨酶(ADA)及其同工酶(ADA-1和ADA-2)、二肽基肽酶IV (DPPIV - CD26)、外泌5′-核苷酸酶(5′- nc)进行了检测。结果:在患者中发现嘌呤代谢的变化,其严重程度和病理生理意义取决于结核的临床形式,即从轻重专一的过程。ADA单核细胞活性的降低伴随着FCPT患者CD26表达的降低和IPT外肽酶患者的生长,即CD26单核细胞和中性粒细胞的浓度与肺结核的形式有关。另一种嘌呤代谢酶- 5′- nc水平升高在两种形式的肺结核中均有记录。结论:在ADA和CD26与IPT相关的情况下,可以假设它们各自参与细胞增殖和细胞因子产生的激活。低水平的CD26免疫细胞与ADA活性无关,这是FCPT患者的典型特征,反映了他们固有的细胞免疫功能失败。我们可以假设,对于新诊断的IPT,与ADA外肽酶(CD26和5′-NC)的复合物的形成提供了CD26_ADA胞外/胞内腺苷和5′-NC /腺苷的平衡,从而为免疫活性细胞提供了足够的代谢。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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