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{"title":"Lewis Rat Model of Experimental Autoimmune Encephalomyelitis","authors":"Kalliopi Pitarokoili, Bjoern Ambrosius, Ralf Gold","doi":"10.1002/cpns.36","DOIUrl":null,"url":null,"abstract":"<p>In this unit, we describe in detail the most common methods used to break immunological tolerance for central myelin antigens and induce experimental autoimmune encephalomyelitis (EAE) in Lewis rats as an animal model of multiple sclerosis. The resulting disease course ranges from an acute monophasic disease to a chronic relapsing or chronic progressive course, which strongly resembles the human disease. These models enable the study of cellular and humoral autoimmunity against major antigenic epitopes of the myelin basic protein, myelin oligodendrocyte glycoprotein, or proteolipid protein. We provide an overview of common immunization protocols for induction of active and passive EAE, assessment and analysis of clinical score, preparation and purification of myelin basic protein, and derivation of neuroantigen-specific rat T cell lines. Finally, we describe the major clinical characteristics of these models. © 2017 by John Wiley & Sons, Inc.</p>","PeriodicalId":40016,"journal":{"name":"Current Protocols in Neuroscience","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2018-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpns.36","citationCount":"10","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Protocols in Neuroscience","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cpns.36","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Neuroscience","Score":null,"Total":0}
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Abstract
In this unit, we describe in detail the most common methods used to break immunological tolerance for central myelin antigens and induce experimental autoimmune encephalomyelitis (EAE) in Lewis rats as an animal model of multiple sclerosis. The resulting disease course ranges from an acute monophasic disease to a chronic relapsing or chronic progressive course, which strongly resembles the human disease. These models enable the study of cellular and humoral autoimmunity against major antigenic epitopes of the myelin basic protein, myelin oligodendrocyte glycoprotein, or proteolipid protein. We provide an overview of common immunization protocols for induction of active and passive EAE, assessment and analysis of clinical score, preparation and purification of myelin basic protein, and derivation of neuroantigen-specific rat T cell lines. Finally, we describe the major clinical characteristics of these models. © 2017 by John Wiley & Sons, Inc.
实验性自身免疫性脑脊髓炎Lewis大鼠模型
在本单元中，我们详细描述了用于破坏对中枢髓磷脂抗原的免疫耐受并诱导Lewis大鼠作为多发性硬化症动物模型的实验性自身免疫性脑脊髓炎(EAE)的最常用方法。由此产生的疾病过程范围从急性单相疾病到慢性复发或慢性进展过程，这与人类疾病非常相似。这些模型能够研究针对髓鞘碱性蛋白、髓鞘少突胶质细胞糖蛋白或蛋白脂质的主要抗原表位的细胞和体液自身免疫。我们概述了用于诱导主动和被动EAE的常见免疫方案，临床评分评估和分析，髓鞘碱性蛋白的制备和纯化以及神经抗原特异性大鼠T细胞系的衍生。最后，我们描述了这些模型的主要临床特征。©2017 by John Wiley &儿子,Inc。
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