{"title":"Genetic Investigation of Beta-Lactam Associated Antibiotic Resistance Among <i>Escherichia Coli</i> Strains Isolated from Water Sources.","authors":"Reza Ranjbar, Mehrdad Sami","doi":"10.2174/1874285801711010203","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Antimicrobial resistance is an important factor threatening human health. It is widely accepted that antibiotic resistant bacteria such as <i>Escherichia coli</i> (<i>E. coli)</i> released from humans and animals into the water sources, can introduce their resistance genes into the natural bacterial community.</p><p><strong>Objective: </strong>The aim of this study was to investigate the prevalence of <i>bla<sub>TEM</sub>, bla<sub>CTX</sub>, bla<sub>SHV</sub>, bla<sub>OXA</sub></i> and <i>bla<sub>VEB</sub></i> associated-antibiotic resistance among <i>E. coli</i> bacteria isolated from different water resources in Iran.</p><p><strong>Methods: </strong>The study contained all <i>E. coli</i> strains segregated from different surface water sources. The Kirby-Bauer method and combined discs method was determined in this study for testing antimicrobial susceptibility and strains that produced Extended-Spectrum Beta Lactamases (ESBL), respectively. DNA extraction kit was applied for genomic and plasmid DNA derivation. Finally the frequency of resistant genes including <i>bla<sub>TEM</sub>, bla<sub>CTX</sub>, bla<sub>SHV</sub>, bla<sub>OXA</sub></i> and <i>bla<sub>VEB</sub></i> in ESBL producing isolates were studied by PCR.</p><p><strong>Results: </strong>One hundred <i>E. coli</i> strains were isolated and entered in the study. The highest antibiotic resistance was observed on clindamycin (96%). Moreover, 38.5% isolates were ESBL producers. The frequency of different ESBLs genes were 37%, 27%, 27%, and 25% for <i>bla<sub>TEM</sub>, bla<sub>CTX</sub>, bla<sub>SHV</sub>, and bla<sub>OXA</sub></i> , respectively. The <i>bla<sub>VEB</sub></i> wasn't found in any isolates.</p><p><strong>Conclusion: </strong>The study revealed a high prevalence of <i>CTX-M, TEM, SHV</i> and <i>OXA</i> genes among <i>E. coli</i> strains in surface water resources. In conclusion, these results raised a concern regarding the presence and distribution of these threatening factors in surface water sources and its subsequent outcomes.</p>","PeriodicalId":38953,"journal":{"name":"Open Microbiology Journal","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2017-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2174/1874285801711010203","citationCount":"16","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Open Microbiology Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2174/1874285801711010203","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2017/1/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"Immunology and Microbiology","Score":null,"Total":0}
引用次数: 16
Abstract
Background: Antimicrobial resistance is an important factor threatening human health. It is widely accepted that antibiotic resistant bacteria such as Escherichia coli (E. coli) released from humans and animals into the water sources, can introduce their resistance genes into the natural bacterial community.
Objective: The aim of this study was to investigate the prevalence of blaTEM, blaCTX, blaSHV, blaOXA and blaVEB associated-antibiotic resistance among E. coli bacteria isolated from different water resources in Iran.
Methods: The study contained all E. coli strains segregated from different surface water sources. The Kirby-Bauer method and combined discs method was determined in this study for testing antimicrobial susceptibility and strains that produced Extended-Spectrum Beta Lactamases (ESBL), respectively. DNA extraction kit was applied for genomic and plasmid DNA derivation. Finally the frequency of resistant genes including blaTEM, blaCTX, blaSHV, blaOXA and blaVEB in ESBL producing isolates were studied by PCR.
Results: One hundred E. coli strains were isolated and entered in the study. The highest antibiotic resistance was observed on clindamycin (96%). Moreover, 38.5% isolates were ESBL producers. The frequency of different ESBLs genes were 37%, 27%, 27%, and 25% for blaTEM, blaCTX, blaSHV, and blaOXA , respectively. The blaVEB wasn't found in any isolates.
Conclusion: The study revealed a high prevalence of CTX-M, TEM, SHV and OXA genes among E. coli strains in surface water resources. In conclusion, these results raised a concern regarding the presence and distribution of these threatening factors in surface water sources and its subsequent outcomes.
期刊介绍：
The Open Microbiology Journal is a peer-reviewed open access journal which publishes research articles, reviews/mini-reviews, case studies, guest edited thematic issues and short communications/letters covering theoretical and practical aspects of Microbial systematics, evolutionary microbiology, immunology, virology, parasitology , bacteriology, mycology, phycology, protozoology, microbial ecology, molecular biology, microbial physiology, biochemistry, microbial pathogenesis, host-microbe interaction, systems microbiology, synthetic microbiology, bioinformatics. The Open Microbiology Journal , a peer-reviewed journal, is an important and reliable source of current information on developments in the field. The emphasis will be on publishing quality papers rapidly and freely available to researchers worldwide.
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