Acute diarrhoea due to a Shiga toxin 2e-producing Escherichia coli O8 : H19.

JMM case reports Pub Date : 2017-06-14 eCollection Date: 2017-06-01 DOI:10.1099/jmmcr.0.005099
Angela Saupe, Birgit Edel, Wolfgang Pfister, Bettina Löffler, Ralf Ehricht, Jürgen Rödel
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引用次数: 10

Abstract

Introduction. Identification of non-O157 Shiga-toxin-producing Escherichia coli (STEC) infections may be underestimated in microbiological diagnosis. Case presentation. A 58-year-old woman developed diarrhoea with watery and subsequently mucous stool. Initial multiplex PCR testing revealed a positive result for stx2 . Culture isolation of a STEC was successful only after repeated inoculation of chromogenic E. coli media. Molecular characterization was performed and identified the isolate as stx2e-positive STEC of serotype O8 : H19. The strain harboured lpfA, but not eae. Conclusion. This case highlights the usefulness of initial multiplex PCR for diagnosis of non-O157 STEC infection.

由产志贺毒素2的大肠杆菌O8: H19引起的急性腹泻。
介绍。非o157产志贺毒素大肠杆菌(STEC)感染的鉴定在微生物学诊断中可能被低估。案例演示。一名58岁妇女出现腹泻,随后出现水样便和粘液便。初步多重PCR检测显示stx2阳性。只有反复接种显色大肠杆菌培养基才能成功培养分离出产志毒素大肠杆菌。分子鉴定鉴定该分离株为血清型O8: H19 stx2e阳性产志贺毒素大肠杆菌。该菌株含有lpfA,但不含eae。结论。本病例强调了初始多重PCR对诊断非o157产志在大肠杆菌感染的有用性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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