Angela Saupe, Birgit Edel, Wolfgang Pfister, Bettina Löffler, Ralf Ehricht, Jürgen Rödel
{"title":"Acute diarrhoea due to a Shiga toxin 2e-producing <i>Escherichia coli</i> O8 : H19.","authors":"Angela Saupe, Birgit Edel, Wolfgang Pfister, Bettina Löffler, Ralf Ehricht, Jürgen Rödel","doi":"10.1099/jmmcr.0.005099","DOIUrl":null,"url":null,"abstract":"<p><p><b>Introduction.</b> Identification of non-O157 Shiga-toxin-producing <i>Escherichia coli</i> (STEC) infections may be underestimated in microbiological diagnosis. <b>Case presentation.</b> A 58-year-old woman developed diarrhoea with watery and subsequently mucous stool. Initial multiplex PCR testing revealed a positive result for <i>stx<sub>2</sub></i> . Culture isolation of a STEC was successful only after repeated inoculation of chromogenic <i>E. coli</i> media. Molecular characterization was performed and identified the isolate as <i>stx</i><sub>2e</sub>-positive STEC of serotype O8 : H19. The strain harboured <i>lpfA</i>, but not <i>eae</i>. <b>Conclusion.</b> This case highlights the usefulness of initial multiplex PCR for diagnosis of non-O157 STEC infection.</p>","PeriodicalId":73559,"journal":{"name":"JMM case reports","volume":"4 6","pages":"e005099"},"PeriodicalIF":0.0000,"publicationDate":"2017-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5630968/pdf/","citationCount":"10","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"JMM case reports","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1099/jmmcr.0.005099","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2017/6/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 10
Abstract
Introduction. Identification of non-O157 Shiga-toxin-producing Escherichia coli (STEC) infections may be underestimated in microbiological diagnosis. Case presentation. A 58-year-old woman developed diarrhoea with watery and subsequently mucous stool. Initial multiplex PCR testing revealed a positive result for stx2 . Culture isolation of a STEC was successful only after repeated inoculation of chromogenic E. coli media. Molecular characterization was performed and identified the isolate as stx2e-positive STEC of serotype O8 : H19. The strain harboured lpfA, but not eae. Conclusion. This case highlights the usefulness of initial multiplex PCR for diagnosis of non-O157 STEC infection.