Laboratory Evaluation of Residual Efficacy of Actellic 300 CS (Pirimiphos-Methyl) and K-Othrine WG 250 (Deltamethrin) on Different Indoor Surfaces.

Abstract

The nature and type of local indoor resting wall surfaces to certain level influences the residual bio-efficacy of insecticides used in indoor residual spraying programs. Knockdown and mortality effects of an organophosphate Actellic 300 CS and pyrethroid K-Othrine WG 250 insecticides on the field-collected Culex quinquefasciatus were assessed bimonthly from July to November 2014, using World Health Organization (WHO) cones bioassay test. Knockdown and mortality rates were subjected to statistical analysis using χ² and Student t tests. Result of the bioassay test on C quinquefasciatus showed that plywood surfaces had the best residual knockdown activity of Actellic 300 CS with knockdown rate above the WHO-recommended threshold limit of ≥95% for 30 days after treatment. This was followed by mud surface with knockdown rates ≥95% threshold limit 15 days (97%) after treatment. The lowest knockdown rates of less than 95% were observed on cement surface throughout the assessment period. However, the knockdown rates of mosquitoes on deltamethrin WG 250-treated cement and plywood surfaces were 100% and ≥95%, respectively, at 30 days after treatment. But the knockdown activity was below the recommended threshold limit on mud surface during the 17 weeks trial. Knockdown activities varied significantly (p < .05), and it is a function of exposure periods, different surfaces, and insecticide formulations. The 24-hour mortality rates of Actellic 300 CS and K-Othrine WG 250 at 120 days after treatment were 83.6% and 86.7%, and 80% and 83.3%, on plywood and cement surfaces, respectively. A maximum residual period of 75 and 45 days were recorded for Actellic 300 CS and K-Othrine WG 250, respectively, on mud surface. Both Actellic 300 CS and K-Othrine 250 WG were highly effective against Culex mosquito. The extended residual activity of p-methyl CS compared with deltamethrin WG 250 makes it a suitable alternative insecticide against pyrethroid-resistant mosquitoes in Southwest Nigeria.